Cytokeratin 13, Cytokeratin 17, and Ki-67 Expression in Human Acquired Cholesteatoma and Their Correlation With Its Destructive Capacity

Mahmood A. Hamed; Seiichi Nakata; Kazuya Shiogama; Kenji Suzuki; Ramadan H. Sayed; Yoichi Nishimura; Noboru Iwata; Kouhei Sakurai; Badawy S. Badawy; Ken-ichi Inada; Hayato Tsuge; Yutaka Tsutsumi

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자료유형: 학술저널

저자정보: Mahmood A. Hamed Seiichi Nakata Kazuya Shiogama Kenji Suzuki Ramadan H. Sayed Yoichi Nishimura Noboru Iwata Kouhei Sakurai Badawy S. Badawy Ken-ichi Inada Hayato Tsuge Yutaka Tsutsumi

저널정보: 대한이비인후과학회 Clinical and Experimental Otorhinolaryngology Clinical and Experimental Otorhinolaryngology 제10권 제3호

발행연도: 2017.1

수록면: 213 - 220 (8page)

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Objectives. Cholesteatoma is a nonneoplastic destructive lesion of the temporal bone with debated pathogenesis and bone resorptive mechanism. Both molecular and cellular events chiefly master its activity. Continued research is necessary to clarify factors related to its aggressiveness. We aimed to investigate the expression of Ki-67, cytokeratin 13 (CK13) and cytokeratin 17 (CK17) in acquired nonrecurrent human cholesteatoma and correlate them with its bone destructive capacity. Methods. A prospective quantitative immunohistochemical study was carried out using fresh acquired cholesteatoma tissues (n=19), collected during cholesteatoma surgery. Deep meatal skin tissues from the same patients were used as control (n=8). Cholesteatoma patients were divided into 2 groups and compared (invasive and noninvasive) according to a grading score for bone resorption based upon clinical, radiologic and intraoperative findings. To our knowledge, the role of CK17 in cholesteatoma aggressiveness was first investigated in this paper. Results. Both Ki-67 and CK17 were significantly overexpressed in cholesteatoma than control tissues (P<0.001 for both Ki-67 and CK17). In addition, Ki-67 and CK17 were significantly higher in the invasive group than noninvasive group of cholesteatoma (P=0.029, P=0.033, respectively). Furthermore, Ki-67 and CK17 showed a moderate positive correlation with bone erosion scores (r=0.547, P=0.015 and r=0.588, P=0.008, respectively). In terms of CK13, no significant difference was found between cholesteatoma and skin (P=0.766). Conclusion. Both Ki-67 and CK17 were overexpressed in cholesteatoma tissue and positively correlated with bone resorption activity. The concept that Ki-67 can be a predictor for aggressiveness of cholesteatoma was supported. In addition, this is the first study demonstrating CK17 as a favoring marker in the aggressiveness of acquired cholesteatoma.

#Cholesteatoma #Middle Ear #Bone Resorption #Ki-67 Antigen #Cytokeratin 17

참고문헌 (30)

참고문헌 신청

Kim HJ / 2002 / Increased proliferation and migration of epithelium in advancing experimental cholesteatomas / Otol Neurotol 23(6):840~844

Lee RJ / 1994 / The nature of the epithelium in acquired cholesteatoma: part 3: cytokeratin patterns in aural epithelial and cholesteatoma cells grown in cell culture / Clin Otolaryngol Allied Sci 19(6):516~520

Toyoshima T / 2008 / Cytokeratin 17 mRNA expression has potential for diagnostic marker of oral squamous cell carcinoma / J Cancer Res Clin Oncol 134(4):515~521

Mikami T / 2011 / Emergence of keratin 17 vs. loss of keratin 13: their reciprocal immunohistochemical profiles in oral carcinoma in situ / Oral Oncol 47(6):497~503

Jin L / 2014 / Keratin 17: a critical player in the pathogenesis of psoriasis / Med Res Rev 34(2):438~454

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