Expression of Insulin-like Growth Factors in a Mouse Model of Salicylate Ototoxicity

임기정; 최준; 장지원; Seo Jin Kim; Hye In Kim; 정학현

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자료유형: 학술저널

저자정보: 임기정 최준 장지원 Seo Jin Kim Hye In Kim 정학현

저널정보: 대한이비인후과학회 Clinical and Experimental Otorhinolaryngology Clinical and Experimental Otorhinolaryngology 제3권 제3호

발행연도: 2010.1

수록면: 115 - 121 (7page)

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Objectives. Insulin-like growth factors (IGFs) are known to be neurotrophic factors, and they efficiently signal to cells to grow, differentiate and survive. The purpose of study was to identify the expressions of IGFs in mice with salicylate ototoxicity, which is a typical reversible hearing loss model. Methods. The mice were given intraperitoneal injections of salicylate (400 mg/kg) and about a 30 dB threshold shift was achieved at 3 hours. The expressions of IGF-1 and 2 were confirmed by semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) and Western blotting. Localization of IGFs was confirmed using confocal immunofluorescence imaging. For in-vitro study on the HEI-OC1 auditory cells, the cell viability was calculated and the apoptotic features of the nuclei were observed with Hoechst staining. Results. The expressions of the IGFs mRNA and protein were significantly increased in the salicylate ototoxicity groups compared with that of the normal control group. Salicylate induced apoptosis and decreased viability of the HEI-OC1auditory cells in a time- and dose-dependent manner. The expressions of IGFs were localized in the stria vascularis,and these IGFs play a protective role in the in-vivo condition of salicylate ototoxicity. Conclusion. IGFs were highly expressed in the mice with salicylate ototoxicity, and this expression was mainly focused in the stria vascularis in the salicylate intoxicated mice. The systemic action of IGFs, which were expressed in the vascular-rich stria vascularis, can act as a major protective mechanism in a mouse model of salicylate ototoxicity.

#Insulin-like growth factor #Salicylate #Ototoxicity #Cochlea

참고문헌 (20)

참고문헌 신청

Biddle C / 1988 / Insulin-like growth factors and the multiplication of Tera-2, a human teratoma-derived cell line / J Cell Sci 90 (Pt 3) : 475 ~ 484

Burns JL / 2001 / Cell survival and proliferation are modified by insulin-like growth factor 2 between days 9 and 10 of mouse gestation / Development 128 (19) : 3819 ~ 3830

Camarero G / 2001 / Delayed inner ear maturation and neuronal loss in postnatal Igf-1-deficient mice / J Neurosci 21 (19) : 7630 ~ 7641

Camarero G / 2002 / Cochlear abnormalities in insulin-like growth factor-1 mouse mutants / Hear Res 170 (1-2) : 2 ~ 11

Cazals Y / 2000 / Auditory sensori-neural alterations induced by salicylate / Prog Neurobiol 62 (6) : 583 ~ 631

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