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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2005.1
- 수록면
- 579 - 583 (5page)
이용수
초록· 키워드
오류제보하기
In this study, the cytotoxicity of medical latex gloves to cultured L-929 cells was determined using various extraction conditions. According to the extraction time and temperature, three types of extraction conditions were used: 1) 24 h at 37℃; 2) 72 h at 37℃; 3) 72 h at 50℃. Also, four different extraction vehicles were used, namely, distilled water (DW), 9g/l sodium chloride (saline) in DW, and culture media with or without serum. Under the above-mentioned conditions, the samples were extracted and then 2-fold serially diluted in the concentration range 3.13-50%. When extracted with either DW or saline for 24h or 72 h at 37℃, only 50% diluted samples showed distinct cytotoxicity to L-929 cells. Moreover, no cytotoxic potentials were observed when gloves were extracted with DW or saline at 50℃ for 72 h. Cytotoxicity was markedly greater when gloves were extracted with culture medium, irrespective of the presence of serum in the medium. These results suggest that optimal extraction conditions should be established for the cytotoxicity evaluations of biomaterials and medical devices.
목차
등록된 정보가 없습니다.
참고문헌 (25)
참고문헌 신청
/ / Comparative studies by cell culture and in vivo implantation test on the toxicity of natural rubber latex materials : 339 ~ 56
/ / Comparative studies of the toxicity of standard reference materials in various cytotoxicity tests and in vivo implantation tests : 153 ~ 6
/ / Improved sensitivity and decreased sample size in a cytotoxicity test for biomaterials: a modified color microassay using a microplate and crystal violet staining : 361
/ / In vivo validation of a cell culture test for biocompatibility testing of urinary catheters : 1125 ~ 35
/ / Studies on the standardization of cytotoxicity tests and new standard reference materials useful for evaluation the safety of biomaterials : 138 ~ 57
/ / Comparative studies of the toxicity of standard reference materials in various cytotoxicity tests and in vivo implantation tests : 153 ~ 6
/ / Improved sensitivity and decreased sample size in a cytotoxicity test for biomaterials: a modified color microassay using a microplate and crystal violet staining : 361
/ / In vivo validation of a cell culture test for biocompatibility testing of urinary catheters : 1125 ~ 35
/ / Studies on the standardization of cytotoxicity tests and new standard reference materials useful for evaluation the safety of biomaterials : 138 ~ 57
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