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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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Transcriptional enhancer activation domain (TEAD) proteins are transcription factors that promote the expression ofgenes involved in organogenesis, embryonic development, and tumorigenesis. TEAD functions downstream of the Hipposignaling pathway as one of the pivotal regulators that controls cell viability and proliferation, tissue growth, and organsize, by interacting with yes-associated protein (YAP) via its C-terminal YAP-binding domain (YBD). As YAP is a wellknownoncoprotein and its interaction with TEAD has been shown to be critical for the function of YAP, TEAD proteinsare emerging as a potential therapeutic target for cancer. In this study, the YBD of the TEAD1 protein was producedfrom an Escherichia coli expression system, purified using a Ni-NTA affinity chromatography, HiTrap Q anion exchangechromatography, and size exclusion chromatography, and then successfully crystallized. X-ray diffraction data werecollected to the resolution of 1.70 Å. Preliminary diffraction analysis revealed that the TEAD1 YBD crystals belong to thespace group P212121 with unit cell parameters of a = 36.5 Å, b = 89.4 Å, c = 135.6 Å, and that two TEAD1 YBD moleculesare contained in the asymmetric unit with a solvent content of 45.2%.
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참고문헌 (24)
참고문헌 신청
Chen, L / 2010 / Structural basis of YAP recognition by TEAD4 in the hippo pathway / Genes Dev 24:290~300
Harvey, K. F / 2013 / The Hippo pathway and human cancer / Nat Rev Cancer 13:246~257
Holden, J. K / 2018 / Targeting the Hippo pathway and cancer through the TEAD family of transcription factors / Cancers 10:81
Huang, J / 2005 / The Hippo signaling pathway coordinately regulates cell proliferation and apoptosis by inactivating Yorkie, the Drosophila Homolog of YAP / Cell 122:421~434
Jiao, S / 2014 / A peptide mimicking VGLL4 function acts as a YAP antagonist therapy against gastric cancer / Cancer Cell 25:166~180
Harvey, K. F / 2013 / The Hippo pathway and human cancer / Nat Rev Cancer 13:246~257
Holden, J. K / 2018 / Targeting the Hippo pathway and cancer through the TEAD family of transcription factors / Cancers 10:81
Huang, J / 2005 / The Hippo signaling pathway coordinately regulates cell proliferation and apoptosis by inactivating Yorkie, the Drosophila Homolog of YAP / Cell 122:421~434
Jiao, S / 2014 / A peptide mimicking VGLL4 function acts as a YAP antagonist therapy against gastric cancer / Cancer Cell 25:166~180
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