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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2006.1
- 수록면
- 265 - 272 (8page)
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Phosphoinositide-specific phospholipase C-γ1 (PLC- γ1) has two pleckstrin homology (PH) domains: an amino-terminal domain (PH1) and a split PH domain (PH2). Here, we show that overlay assay of bovine brain tubulin pool with glutathione-S-transferase (GST)-PLC-γ1 PH domain fusion proteins, followed by matrix-assisted laser-desorption ionization-time fied 68-kDa neurofilament light chain (NF-L) as a binding protein of amino-terminal PH domain of PLC-γ1. NF-L is known as a component of neuronal intermediate filaments, which are responsible for supporting the structure of myelinated axons in neuron. PLC-γ1 and NF-L colocalized in the neurite in PC12 cells upon nerve growth factor stimulation. In vitro binding assay and immunoprecipitation analysis also showed a specific interaction of both proteins in differentiated PC12 cells. The phospha-tidylinositol 4, 5-bisphosphate [PI(4,5)P2] hydrolyz-ing activity of PLC-γ1 was slightly decreased in the presence of purified NF-L in vitro, suggesting that NF-L inhibits PLC-γ1. Our results suggest that PLC-γ1-associated NF-L sequesters the phospho-lipid from the PH domain of PLC-γ1.
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참고문헌 (28)
참고문헌 신청
/ / Cleveland DW. Neurofilaments are obligate heteropolymers in vivo. Journal of Cell Biology 1993 : 1337 ~ 50
/ / GTP regeneration influences interactions of microtubules and microtubule- associated proteins in vitro. J Biol Chem 1987 : 15443 ~ 7
/ / Gilman AG. Purification from Sf9 cells and char-acterization of recombinant Gqα and G11α. Activation of puri-fied phospholipase C isozymes by Gα subunits. J Biol Chem
/ / Hirokawa N. Cross-linker system between neurofilaments and membranous organelles in frog axons re-vealed by the quick-freeze : 129 ~ 42
/ / Hirokawa N. Molecular architecture of the neurofilament. II. Reassembly process of neurofilament L pro-tein in vitro. J Mol Biol 1990 : 871 ~ 82
/ / GTP regeneration influences interactions of microtubules and microtubule- associated proteins in vitro. J Biol Chem 1987 : 15443 ~ 7
/ / Gilman AG. Purification from Sf9 cells and char-acterization of recombinant Gqα and G11α. Activation of puri-fied phospholipase C isozymes by Gα subunits. J Biol Chem
/ / Hirokawa N. Cross-linker system between neurofilaments and membranous organelles in frog axons re-vealed by the quick-freeze : 129 ~ 42
/ / Hirokawa N. Molecular architecture of the neurofilament. II. Reassembly process of neurofilament L pro-tein in vitro. J Mol Biol 1990 : 871 ~ 82
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