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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2009.1
- 수록면
- 180 - 188 (9page)
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초록· 키워드
오류제보하기
Tumor cells are known to produce larger amounts of reactive oxygen species (ROS) than normal cells.
Although numerous reports have indicated the importance of ROS in urokinase plasminogen activator (uPA) production, the precise mechanisms remain controversial. In our study, we investigated the effect of ROS on uPA generation in human hepatoma cells, HepG2 and Hep 3B. We determined the effects of hepatocyte growth factor (HGF) on the regulation of ROS, which resulted in suppression of ROS production, as measured with the fluorescent probe, 2'-7'-dichlorofluorescein diacetate. The role of HGF in modulating ROS production, particularly that regulated by Rac-1, was determined. HGF suppressed the increment in Rac-1-regulated ROS in both cell lines. Treatment with 200 μM of H2O2 showed a 1.6-2.1 fold increment in HGF, but a little increment occurred at 500 μM of H2O2. It looks no dose dependent manner. Combined treatment with H2O2 and HGF, resulted in a slightly increased production of HGF compared to no treatment (control).
Also, H2O2 upregulated uPA expression in both hepatoma cell lines. To identify the downstream pathways regulated by ROS, we treated cells with PD 98059, an MEK inhibitor, and SB 203580, a p38 inhibitor, after treatment with H2O2, and showed negative control between ERK and p38 kinase activities for uPA regulation.
We found that HGF modulate Rac-1-regulated ROS production through activation of Akt and ROS regulates uPA production via MAP kinase, which provides a novel clue to clarify the mechanism underlying hepatoma progression.
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등록된 정보가 없습니다.
참고문헌 (23)
참고문헌 신청
Aguirre-Ghiso JA / 2003 / ERKMAPKActivity as a Determinant of Tumor Growth and Dormancy;Regulation by p38SAPK1 / Cancer Research 63 : 1684 ~ 1695
Andreasen PA / 1997 / The urokinase-type plasminogen activator system in cancer metastasis: a review / Int J Cancer 72 : 1 ~ 22
Aslan M / 2003 / Oxidants in receptor tyrosine kinase signal transduction pathways / Antioxidants & Redox Signalling 5 : 781 ~ 788
Berra E / 1995 / Evidence for a role of MEK and MAPK during signal transduction by rotein kinase C zeta / EMBO Journal 14 : 6157 ~ 6163
Blasi F / 1996 / The urokinase receptor and cell migration / Semin Thromb Hemost 22 : 513 ~ 516
Andreasen PA / 1997 / The urokinase-type plasminogen activator system in cancer metastasis: a review / Int J Cancer 72 : 1 ~ 22
Aslan M / 2003 / Oxidants in receptor tyrosine kinase signal transduction pathways / Antioxidants & Redox Signalling 5 : 781 ~ 788
Berra E / 1995 / Evidence for a role of MEK and MAPK during signal transduction by rotein kinase C zeta / EMBO Journal 14 : 6157 ~ 6163
Blasi F / 1996 / The urokinase receptor and cell migration / Semin Thromb Hemost 22 : 513 ~ 516
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