지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2005.1
- 수록면
- 91 - 100 (10page)
이용수
초록· 키워드
오류제보하기
The calcium sensing receptor (CaSR) plays an important role for sensing local changes in the extracellular calcium concentration ([Ca2+]o) in bone remodeling. Although the function of CaSR is known, the regulatory mechanism of CaSR remains controversial. Therefore, we report here the regulatory effect of caveolin on CaSR function as a process of CaSR regulation by using the human osteosarcoma cell line (Saos-2). The intracellular calcium concentration ([Ca2+]i) was increased by an increment of [Ca2+]o. This [Ca2+]i increment was inhibited by the pretreatment with NPS 2390, an antagonist of CaSR. RT-PCR and Western blot analysis of Saos-2 cells revealed the presence of CaSR, caveolin (Cav)-1 and –2 in both mRNA and protein expressions, but there was no expression of Cav-3 mRNA and protein in the cells. In the isolated caveolae-rich membrane fraction from Saos-2 cells, the CaSR, Cav-1 and Cav-2 proteins were localized in same fractions (fraction number 4 and 5). The immuno-precipitation experiment using the respective antibodies showed complex formation between the CaSR and Cav-1, but not complex formation of CaSR and Cav-2. Confocal microscopy also supported the co-localization of CaSR and Cav-1 at the plasma membrane. Functionally, the [Ca2+]o-induced [Ca2+]i increment was attenuated by the introduction of Cav-1 antisense oligodeoxynucleotide (ODN). From these results, in Saos-2 cells, the function of CaSR might be regulated by binding with Cav-1. Considering the decrement of CaSR activity by antisense ODN, Cav-1 up-regulates the function of CaSR under normal physiological conditions, and it may play an important role in the diverse pathophysiological processes of bone remodeling or in the CaSR-related disorders in the body.
목차
등록된 정보가 없습니다.
참고문헌 (22)
참고문헌 신청
/ / Brown EM. Decreased expression of caveolin-1 and alteredregulation of mitogen-activated protein kinase in culturedbovine parathyroid cells and human parathyroid adenomas
/ / Brown EM. The calciumsensingreceptor is localized in caveolin-rich plasma membranedomains of bovine parathyroid cells. J Biol Chem1998 : 21708 ~ 13
/ / Brown EM.m-Calpain colocalizes with the calcium-sensing receptor in caveolae in parathyroid cells and participates indegradation of the CaR. J Biol Chem 2003a : 31167 ~
/ / Casanova JE.Evidence for a regulated interaction between heterotrimericG proteins and caveolin. J Biol Chem 1995 : 15693 ~ 701
/ / CorderaR. IGF-I induces caveolin 1 tyrosine phosphorylation andtranslocation in the lipid rafts. Biochem Biophys Res Commun2002 : 1085 ~ 9
/ / Brown EM. The calciumsensingreceptor is localized in caveolin-rich plasma membranedomains of bovine parathyroid cells. J Biol Chem1998 : 21708 ~ 13
/ / Brown EM.m-Calpain colocalizes with the calcium-sensing receptor in caveolae in parathyroid cells and participates indegradation of the CaR. J Biol Chem 2003a : 31167 ~
/ / Casanova JE.Evidence for a regulated interaction between heterotrimericG proteins and caveolin. J Biol Chem 1995 : 15693 ~ 701
/ / CorderaR. IGF-I induces caveolin 1 tyrosine phosphorylation andtranslocation in the lipid rafts. Biochem Biophys Res Commun2002 : 1085 ~ 9
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Co-localization and interaction of organic anion transporter 1 with caveolin-2 in rat kidney
Experimental and Molecular Medicine
2005 .01
-
Caveolin-1 regulates osteoclast differentiation by suppressing cFms degradation
Experimental and Molecular Medicine
2015 .01
-
A Dinucleotide Repeat polymorphism at the Calcium Sensing Recepator(CASR) locus in Korean Pupulation
한국동물학회 학술대회
2000 .01
-
Co-localization and interaction of human organic anion transporter 4 with caveolin-1 in primary cultured human placental trophoblasts
Experimental and Molecular Medicine
2008 .01
-
Asn-Linked Glycosylation Contributes to Surface Expression and Voltage-Dependent Gating of Cav1.2 Ca2+ Channel
Journal of Microbiology and Biotechnology
2015 .01
-
Antisense Heat Shock Gene 도입에 따른 식물세포의 변화
한국식물학회 심포지움
1993 .01
-
Caveolin-1 upregulation in senescent neurons alters amyloid precursor protein processing
Experimental and Molecular Medicine
2006 .01
-
Photo-controlled gene expression by fluorescein-labeled antisense oligonucleotides in combination with visible light irradiation
Journal of photoscience : an international journal officail organ of the korean society of photoscience
2002 .01
-
Regulation of Cav3.2 Ca Channel Activity by Protein Tyrosine Phosphorylation
Journal of Microbiology and Biotechnology
2008 .01
-
Role of caveolin in the regulation of cell cycle by insulin
한국생명과학회 심포지움
2002 .12
-
Structural Characterization for N-Terminal Domain of Caveolin-l
Korean journal of biological sciences
2003 .01
-
Development of an Indirect ELISA Featuring Plates Coated with Column Chromatographically Purified Canine Adenovirus Type-1 Antigen
JOURNAL OF BACTERIOLOGY AND VIROLOGY
2020 .03
-
Effect of Human Cytomegalovirus (HCMV) Replication on the Production of Alkaline Phosphatase in Osteosarcoma Cell Line (Saos-2)
JOURNAL OF BACTERIOLOGY AND VIROLOGY
2002 .03
-
Antisense Technology를 이용한 탄저균 치사 독소 발현 억제
JOURNAL OF BACTERIOLOGY AND VIROLOGY
2004 .12
-
Antisense RNA : A new solution to a traditional problem in dairy starter cultures
생명과학
1994 .01
-
Synergistic effect of peroxiredoxin II antisense on cisplatininduced cell death
Experimental and Molecular Medicine
2002 .01
-
Effect of Intracerebroventricular Administration of an Antisense Oligodeoxynucleotide against Type I Receptor of Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP) on the Pituitary Luteinizing hormone $\beta$ messenger RNA level
한국동물학회 학술대회
1999 .01
-
Analysis of Gene Expression in Cyclooxygenase-2-Overexpressed Human Osteosarcoma Cell Lines
Genomics & informatics
2014 .01
-
HPV E6 antisense induces apoptosis in CaSki cells via suppression of E6 splicing
Experimental and Molecular Medicine
2002 .01
-
The Response to a Various Stresses in Transgenic Tobacco Plants Expressing the Antisense Genes of Biosynthesis for Ethylene
한국동물학회 학술대회
2003 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0