지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제25권 제8호
- 발행연도
- 2015.1
- 수록면
- 1,371 - 1,379 (9page)
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초록· 키워드
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The Cav1.2 Ca2+ channel is essential for cardiac and smooth muscle contractility and many physiological functions. We mutated single, double, and quadruple sites of the four potential Asn (N)-glycosylation sites in the rabbit Cav1.2 into Gln (Q) to explore the effects of Nglycosylation. When a single mutant (N124Q, N299Q, N1359Q, or N1410Q) or Cav1.2/WT was expressed in Xenopus oocytes, the biophysical properties of single mutants were not significantly different from Cav1.2/WT. In comparison, the double mutant N124,299Q showed a positive shift in voltage-dependent gating. Furthermore, the quadruple mutant (QM; N124,299,1359,1410Q) showed a positive shift in voltage-dependent gating as well as a reduction of current. We tagged EGFP to the QM, double mutants, and Cav1.2/WT to chase the mechanisms underlying the reduced currents of QM. The surface fluorescence intensity of QM was weaker than that of Cav1.2/WT, suggesting that the reduced current of QM arises from its lower surface expression than Cav1.2/WT. Tunicamycin treatment of oocytes expressing Cav1.2/WT mimicked the effects of the quadruple mutations. These findings suggest that Nglycosylation contributes to the surface expression and voltage-dependent gating of Cav1.2.
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참고문헌 (17)
참고문헌 신청
Bennett ES / 2002 / Isoform-specific effects of sialic acid on voltage-dependent Na+ channel gating: functional sialic acids are localized to the S5-S6 loop of domain I / J.
Catterall WA / 2011 / Voltage-gated calcium channels / Cold Spring Harbor Perspect. Biol 3 : a003947 ~
Egenberger B / 2010 / N-linked glycosylation determines cell surface expression of two-pore-domain K+ channel TRESK / Biochem. Biophys. Res. Commun 391 : 1262 ~ 1267
Eklund EA / 2006 / The congenital disorders of glycosylation: a multifaceted group of syndromes / NeuroRx 3 : 254 ~ 263
Fujita T / 2006 / Glycosylation and cell surface expression of Kv1.2 potassium channel are regulated by determinants in the pore region / Neurochem. Res 31 : 589 ~ 596
Catterall WA / 2011 / Voltage-gated calcium channels / Cold Spring Harbor Perspect. Biol 3 : a003947 ~
Egenberger B / 2010 / N-linked glycosylation determines cell surface expression of two-pore-domain K+ channel TRESK / Biochem. Biophys. Res. Commun 391 : 1262 ~ 1267
Eklund EA / 2006 / The congenital disorders of glycosylation: a multifaceted group of syndromes / NeuroRx 3 : 254 ~ 263
Fujita T / 2006 / Glycosylation and cell surface expression of Kv1.2 potassium channel are regulated by determinants in the pore region / Neurochem. Res 31 : 589 ~ 596
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