메뉴 건너뛰기
.. 내서재 .. 알림
소속 기관/학교 인증
인증하면 논문, 학술자료 등을  무료로 열람할 수 있어요.
한국대학교, 누리자동차, 시립도서관 등 나의 기관을 확인해보세요
(국내 대학 90% 이상 구독 중)
로그인 회원가입 고객센터 ENG
주제분류

추천
검색

이용수

표지
📌
연구주제
📖
연구배경
🔬
연구방법
🏆
연구결과
AI에게 요청하기
추천
검색

초록· 키워드

오류제보하기
How to improve the cell culture method onscaffolds is important in the tissue engineering fileld. In thisstudy, we optimized seeding and culture methods to vascularsmooth muscle cells (VSMCs) on biodegradable polymerscaffold. The primary culture of VSMCs obtained fromcanine external jugular vein was accomplished by applyingthe explant-derived method. The primary cultured VSMCswere seeded into scaffolds and then cultured by using variousdifferent methods; static or dynamic seeding, static or dynamicculture. The difference in proliferative response of VSMCswas analyzed with an alamar blue assay. Cell-polymer constructwas examined by histochemical method and scanning electronmicroscopy. Mesh type scaffold (10×10×0.4 mm) wasmade of polyglycolic acid (PGA) suture thread. The PGAmesh type scaffold was 45% in porosity, and 0.03 g inweight. The primary cultured VSMCs were confirmed withimmunohistochemical staining using monoclonal anti-α-smooth muscle actin. The density and distribution of proliferatedVSMCs within the scaffold and cellular adherence on thesurface of the scaffold showed better results in the staticseeding condition than in the dynamic condition. Under thesame condition of seeding method as the static condition, thedynamic culture condition showed enhanced proliferationrates of the VSMCs when compared to the static culturecondition. In conclusion, to improve the VSMCs proliferationin vitro, static seeding is better than the dynamic condition. Inthe culture condition, however, culture under the dynamicstatus is better than the static condition. This was a pilot studyto manufacture artificial vascular vessel by tissue engineering.

목차

등록된 정보가 없습니다.

참고문헌 (25)

참고문헌 신청

함께 읽어보면 좋을 논문

논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!

이 논문의 저자 정보

최근 본 자료

전체보기

댓글(0)

0