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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제18권 제8호
- 발행연도
- 2008.1
- 수록면
- 1,453 - 1,458 (6page)
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초록· 키워드
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For quantitative PCR assay of Salmonella enterica serovar
Typhimurium in food samples, a real-time PCR method
was developed, based on DNA genome equivalent. Specific
primers and probe designed based on the STM4497 gene
of S. Typhimurium LT2 showed the specificity to S.
Typhimurium. Threshold cycle (Ct) values of real-time PCR
were obtained from a quantitative standard curve with
genomic DNA of Salmonella Typhimurium. In addition,
the recovery of S. Typhimurium inoculated artificially
to chicken samples with 4.5×105 to 4.5 CFU/ml was
evaluated by using real-time PCR and plate-count methods.
Result showed that the number of cells calculated from
the real-time PCR method had good correlation with that
of the plate-count method. This real-time PCR method
could be applicable to the detection and quantification of
S. Typhimurium in food samples.
목차
등록된 정보가 없습니다.
참고문헌 (23)
참고문헌 신청
Baumler, A. J. / 1997 / Rapid detection of Salmonella enterica with primers specific for iroB. J. Clin / Microbiol 35 : 1224 ~ 1230
Bhagwat, A. A. / 2003 / Rapid detection of Salmonella from vegetable rinse-water using real-time PCR / Food Microbiol 21 : 73 ~ 78
Cheung, P. Y. / 2004 / Evaluation of two real-time polymerase chain reaction pathogen detection kits for Salmonella spp. in food. Lett. Appl / Microbiol 39 : 509 ~ 515
Cohen, H. J. / 1996 / PCR amplification of the fimA gene sequence of Salmonella typhimurium, a specific method for detection of Salmonella spp. Appl. Environ / Microbiol 62 :
Eyigor, A. / 2002 / Implementation of real-time PCR to tetrathionate broth enrichment step of Salmonella detection in poultry. Lett. Appl / Microbiol 34 : 37 ~ 41
Bhagwat, A. A. / 2003 / Rapid detection of Salmonella from vegetable rinse-water using real-time PCR / Food Microbiol 21 : 73 ~ 78
Cheung, P. Y. / 2004 / Evaluation of two real-time polymerase chain reaction pathogen detection kits for Salmonella spp. in food. Lett. Appl / Microbiol 39 : 509 ~ 515
Cohen, H. J. / 1996 / PCR amplification of the fimA gene sequence of Salmonella typhimurium, a specific method for detection of Salmonella spp. Appl. Environ / Microbiol 62 :
Eyigor, A. / 2002 / Implementation of real-time PCR to tetrathionate broth enrichment step of Salmonella detection in poultry. Lett. Appl / Microbiol 34 : 37 ~ 41
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