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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제18권 제9호
- 발행연도
- 2008.1
- 수록면
- 1,510 - 1,517 (8page)
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초록· 키워드
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Expresssed sequence tag (EST) analysis was developed from three cDNA libraries constructed from cells of the digestive tract, gonad, and liver of sea squirt. Randomly selected cDNA clones were partially sequenced to generate a total of 922 ESTs, in which 687 unique ESTs were identified respectively. Results of BLASTX search showed that 612 ESTs (89%) have homology to genes of known function whereas 75 ESTs (11%) were unidentified or novel. Based on the major function of their encoded proteins, the identified clones were classified into ten broad categories.
We also identified several kinds of immune-related genes as identifying novel genes. Sequence analysis of ESTs revealed the presence of microsatellite-containing genes that may be valuable for further gene mapping studies.
The accumulation of a large number of identified cDNA clones is invaluable for the study of sea squirt genetics and developmental biology. Further studies using cDNA microarrays are needed to identify the differentially expressed transcripts after disease infection.
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참고문헌 (24)
참고문헌 신청
[학술지(정기간행물)]
Altschul, S. F. / 1997 / Gapped BLAST and PSI-BLAST: A new generation of protein database search program / Nucleic Acids Res 25 : 3389 ~ 3402
Blount, P. / 1991 / BIP associates with newly synthesized subunits of the mouse muscle nicotinic receptor / Cell Biol 113 : 1125 ~ 1132
Carmel-Harel, O. / 2000 / Roles of the glutathioneand thioredoxin-dependent reduction systems in the Escherichia coli and Saccharomyces cerevisiae responses to oxidative stre
Cho, H. K. / 2008 / Identification of softness syndrome-associated candidate genes and DNA sequence variation in the sea squirt, Halocynthia roretzi. Mar / Biotechnol 10 : 44
Altschul, S. F. / 1997 / Gapped BLAST and PSI-BLAST: A new generation of protein database search program / Nucleic Acids Res 25 : 3389 ~ 3402
Blount, P. / 1991 / BIP associates with newly synthesized subunits of the mouse muscle nicotinic receptor / Cell Biol 113 : 1125 ~ 1132
Carmel-Harel, O. / 2000 / Roles of the glutathioneand thioredoxin-dependent reduction systems in the Escherichia coli and Saccharomyces cerevisiae responses to oxidative stre
Cho, H. K. / 2008 / Identification of softness syndrome-associated candidate genes and DNA sequence variation in the sea squirt, Halocynthia roretzi. Mar / Biotechnol 10 : 44
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