지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
An expressed sequence tag (EST) is simply a segment of a sequence over 150 bp from a randomly selected cDNA. EST helps to quickly identify functions of expressed genes and to understand the complexity of gene expression with database comparison. Sequencing of random cDNA clones can be applicable to discovery of new genes, mapping of the genome, identification of coding regions in genomic sequences, and antisense method. To accomplish these goals, in this research, randomly selected cDNA sequencing was performed with watermelon plant. Among 30 clones picked up and analyzed, all clones had an insert length over 0.5 kb. The average size of insert was about 1.3 kb. The size range of most cDNA insert was 1.0-2.0 kb. For sequence comparison, data from the coding region at 5' end of selected cDNA should be much more informative than those from the untranslated 3' tail. Thirty clones were sequenced from one end (5' end). Of these, 29 had no poly (A) tail in this direction, while only one was inverted. Thus, this library is over 96% unidirectonal. Two clones had homologies to ribulose bisphosphate carboxylase/oxigenase (Rubisco) small subunit precursor gene. Thirteen cDNAs had high degree of sequences similarity to genes from other organisms. The remaining cDNA clones seem to be new genes not only in watermelon but also in all orgamisms.
목차
등록된 정보가 없습니다.
참고문헌 (0)
참고문헌 신청함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Characterization of Pathogenesis-Related Gene and Zinc Finger Protein cDNA Clone Obtained by Expressed Sequence Tags of Pimpinella brachycarpa Shoot-tip cDNA
한국동물학회 학술대회
1998 .01
-
Analysis of 176 Expressed Sequence Tags Generated from cDNA Clones of Hot Pepper by Single-pass Sequencing
식물학회지
1998 .01
-
Cloning of the 5'-end and Amplification of Full-Length cDNA of Genomic RNA of Lily symptomless virus
The Plant Pathology Journal
2002 .08
-
Analyses of Expressed Sequence Tags of Anther and Anther-Specific cDNA Clones in Nicotiana tabacum
식물학회지
2000 .01
-
Quantitative Analysis of Expressed Genes in Aspergillus Oryzae by Sequencing 3'-directed cDNA Clones
The Journal of Microbiology
1998 .06
-
Bridging a Gap between DNA sequences and expression patterns of genes
한국생물정보시스템생물학회 심포지엄
2000 .01
-
Isolation and Sequencing of Two Calmodulin cDNA Clones from Rice Flower
한국생명과학회 심포지움
2003 .10
-
Expression of Cold-Stress-inducible cDNA Clone Isolated from Potato (Solanum tuberosum L.) tuber cDNA Library
한국동물학회 학술대회
2000 .01
-
Expressed Sequence Tags of Expression Profiles of Olive Flounder (Paralichthys olivaceus) Testis
한국어류학회지
2007 .01
-
Generation of Expressed Sequence Tags for Immune Gene Discovery and Marker Development in the Sea Squirt, Halocynthia roretzi
Journal of Microbiology and Biotechnology
2008 .01
-
Generation of Full-Length Infectious cDNA Clones of Middle East Respiratory Syndrome Coronavirus
Journal of Microbiology and Biotechnology
2019 .01
-
Sequence of cDNA Clone Corresponding to Lysozyme from Artogeia rapae
한국동물학회 학술대회
1997 .01
-
Quantitative Analysis of Gene Expression Pattern in Aspergillus nidulans Mycelia by Sequencing of 3'-directed cDNA Clones
The Journal of Microbiology
1996 .03
-
Chromosomal Information of 1,144 Korean BAC Clones
Genomics & informatics
2006 .01
-
Characterization and Functional Analysis of Antifungal Protein cDNA Clone from hot Pepper
한국동물학회 학술대회
1999 .01
-
Expressed sequence tags analysis of immune-relevant genes in rock bream Oplegnathus fasciatus gill stimulated with LPS
한국어병학회지
2010 .01
-
Construction and Profiling of a cDNA Library from Young Fruit of Satsuma Mandarin
식물학회지
2007 .01
-
Developing New Mammalian Gene Expression Systems Using the Infectious cDNA Molecular Clone of the Japanese Encephalitis Virus
한국미생물학회 학술대회논문집
2003 .05
-
Analysis of Expressed Sequence Tags Generated from Posterior Silkgland cDNA Clones from Antheraea yamamai
한국응용곤충학회 학술발표회
2000 .01
-
담배 모자이크 바이러스(고추계통) RNA의 cDNA 클로닝 및 유전자진단
The Plant Pathology Journal
1991 .12
이 논문의 저자 정보
최근 본 자료
댓글(0)
0