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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제16권 제6호
- 발행연도
- 2006.1
- 수록면
- 952 - 960 (9page)
이용수
초록· 키워드
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As a unicellular green alga that possesses many of theChlorella offersmany advantages for expresion of heterologous proteins.Since strong and constitutive promoters are necesary forefficient expression in heterologous expression systems, thedevelopment of such promoters for use in the Chlorellasystem was the aim of this study. Proteins encoded by theearly genes of algal viruses are expressed before viral replication,probably by the host transcriptional machinery, and thepromoters of these genes might be useful for heterologousexpresion in Chlorellaof DNA polymerase, ATP-dependent DNA ligase, and chitinasegenes were amplified from eight Korean Chlorela virusisolates by using primer sets designed based on the sequenceof the genome of PBCV-1, the prototype of the Phycodnaviridae.These putative promoter regions were found to containseveral cis-acting elements for transcription factors, includingthe TATA, CAAT, NTBF1, GATA, and CAT boxes.The amplified promoter regions were placed into Chlorella(GFP) reporter gene and the Sh ble gene for phleomycinresistance. C. vulgaris protoplasts were transformed and thenselected with phleomycin. The GFP fluorescence intensitiesof cells transformed with chitinase, DNA polymerase, andDNA ligase gene promoter-GFP fusion constructs were101.5, 100.8, and 95.8%, respectively, of that of CaMV 35S-GFP-transformed Chlorela cells. These results demonstrateChlorela.
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참고문헌 (24)
참고문헌 신청
Baumann, K / 1998 / The DNA binding site of the Dof protein NtBBF1 is essential for tissue-specific and auxin-regulated expression of the rolB oncogene in plants / Plant Cell
Chen, F / 1995 / Amplification of DNA polymerase gene fragments from viruses infecting microalgae / Appl. Environ. Microbiol. 61 : 1274 ~ 1278
Cho, H. H / 2002 / Isolation and characterization of Chlorella viruses from freshwater sources in Korea. / Mol. Cells 142 : 168 ~ 176
Eom, H. S / 2005 / Gene expression profiling of eukaryotic microalga, Haematococcus pluvialis. / J. Microbial. Biotechnol. 15 : 1060 ~ 1066
Grabhrr, R / 1992 / The DNA polymerase gene from Chlorella viruses PBCV-1 and NY 2A contains an intron with nuclear splicing sequences / Virology 188 : 721 ~ 731
Chen, F / 1995 / Amplification of DNA polymerase gene fragments from viruses infecting microalgae / Appl. Environ. Microbiol. 61 : 1274 ~ 1278
Cho, H. H / 2002 / Isolation and characterization of Chlorella viruses from freshwater sources in Korea. / Mol. Cells 142 : 168 ~ 176
Eom, H. S / 2005 / Gene expression profiling of eukaryotic microalga, Haematococcus pluvialis. / J. Microbial. Biotechnol. 15 : 1060 ~ 1066
Grabhrr, R / 1992 / The DNA polymerase gene from Chlorella viruses PBCV-1 and NY 2A contains an intron with nuclear splicing sequences / Virology 188 : 721 ~ 731
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