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Electrochemical control of the metabolic flux ofW. kimchii sk10 on glucose and pyruvate was studied. The growingcell of W. kimchii sk10 produced 87.4 mM lactate, 69.3 mMethanol, and 4.9mM lactate from 83.1mM glucose under oxidationcondition of the anode compartment, but 98.9 mM lactate, 84.3mMethanol, and 0.2 mM acetate were produced from 90.8 mMglucose under reduction condition of the cathode compartmentfor 24 h, respectively. The resting cell of W. kimchii sk10produced 15.9 mM lactate and 15.2 mM acetate from 32.1 mMpyruvate under oxidation condition of the anode compartment,and 71.3 mM lactate and 3.8 mM acetate from 79.8mM pyruvateunder reduction condition of the cathode compartment. Theredox balance (NADH/NAD+) of metabolites electrochemicallyproduced from pyruvate was 1.05 and 18.76 under oxidationand reduction conditions, respectively. On the basis of these results,we suggest that the neutral red (NR) immobilized in bacterialmembrane can function as an electron channel for the electrontransfer between electrode and cytoplasm without dissipationof membrane potential, and that the bacterial fermentation ofW. kimchii sk10 can be shifted to oxidized or reduced pathwaysby the electrochemical oxidation or reduction, respectively.

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