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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제15권 제3호
- 발행연도
- 2005.1
- 수록면
- 525 - 531 (7page)
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초록· 키워드
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From a genomic library of Lactobacillus reuteriATCC 55739, one clone, NE347, carrying a pyrH geneencoding UMP kinase, was identified. pNE347 carried a 1.88 kbEcoRI fragment and the pyrH was located in the middle of theinsert. pyrH ORF was 723 bp in size and capable of encodingUMP kinase composed of 240 amino acid residues. tsfencoding an elongation factor-Ts and frr encoding a ribosomalrecycling factor were present upstream and downstream ofpyrH, respectively. When introduced into E. coli KUR1244, apyrH-negative strain, pNE347 restored the ability to grow at42oC, indicating that pyrH from L. reuteri synthesized functionalUMP kinase in E. coli. Northern blot experiment showed thatpyrH and frr were cotranscribed as a 1.4 kb single transcript.pyrH was overexpressed in E. coli by using a pET26b(+)vector, and a major 25 kDa protein band appeared on SDSpolyacrylamidegel.
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참고문헌 (15)
참고문헌 신청
A / 1992 / nucleotidesequence and expression of the Escherichia coli K-12 pyrHgene encoding UMP kinase : 59 ~ 65
A / 1998 / Mutational analysis of UMP kinasefrom Escherichia coli 180 : 473 ~ 477
Blank / 1996 / Elongationfactor Ts from Thermus thermophilus overproductionin Escherichia coli 236 : 222 ~ 227
Blondin / 1995 / a member of the aspartokinase family isa hexamer regulated by guanine nucleotides and UTP 34 : 5066 ~ 5074
Dower / 1988 / High efficiency transformation of E coli by high voltageelectroporation : 6127 ~ 6145
A / 1998 / Mutational analysis of UMP kinasefrom Escherichia coli 180 : 473 ~ 477
Blank / 1996 / Elongationfactor Ts from Thermus thermophilus overproductionin Escherichia coli 236 : 222 ~ 227
Blondin / 1995 / a member of the aspartokinase family isa hexamer regulated by guanine nucleotides and UTP 34 : 5066 ~ 5074
Dower / 1988 / High efficiency transformation of E coli by high voltageelectroporation : 6127 ~ 6145
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