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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제18권 제1호
- 발행연도
- 2008.1
- 수록면
- 59 - 62 (4page)
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초록· 키워드
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The peptide methionine sulfoxide reductases (Msrs) are enzymes that catalyze the reduction of methionine sulfoxide back to methionine. Because of two enantiomers of methionine sulfoxide (S and R forms), this reduction reaction is carried out by two structurally unrelated classes of enzymes, MsrA (E.C. 1.8.4.11) and MsrB (E.C.
1.8.4.12). Whereas MsrA has been well characterized structurally and functionally, little information on MsrB is available. The recombinant MsrB from Bacillus subtilis has been purified and crystallized by the hanging-drop vapor-diffusion method, and the functional and structural features of MsrB have been elucidated. The crystals belong to the trigonal space group P3, with unit-cell parameters a=b=136.096, c=61.918 , and diffracted to 2.5 resolution using a synchrotron-radiation source at Pohang Light Source. The asymmetric unit contains six subunits of MsrB with a crystal volume per protein mass (VM) of 3.37 Å3 Da-1 and a solvent content of 63.5%.
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참고문헌 (14)
참고문헌 신청
Boschi-Muller, S. / 2000 / A sulfenic acid enzyme intermediate is involved in the catalytic mechanism of peptide methionine sulfoxide reductase from Escherichia coli. J. Biol
Brot, N. / 1981 / Enzymatic reduction of protein-bound methionine sulfoxide. Proc. Natl. Acad / Sci 78 : 2155 ~ 2158
Grimaud, R. / 2001 / Repair of oxidized proteins. Identification of a new methionine sulfoxide reductase. J. Biol / Chem 276 : 48915 ~ 48920
Kryukov, G. V. / 2002 / Selenoprotein R is a zinc-containing stereospecific methionine sulfoxide reductase. Proc. Natl. Acad / Sci 99 : 4245 ~ 4250
Kumar, R. A. / 2002 / Reaction mechanism, evolutionary analysis, and role of zinc in Drosophila methionine-R-sulfoxide reductase. J. Biol / Chem 277 : 37527 ~ 37535
Brot, N. / 1981 / Enzymatic reduction of protein-bound methionine sulfoxide. Proc. Natl. Acad / Sci 78 : 2155 ~ 2158
Grimaud, R. / 2001 / Repair of oxidized proteins. Identification of a new methionine sulfoxide reductase. J. Biol / Chem 276 : 48915 ~ 48920
Kryukov, G. V. / 2002 / Selenoprotein R is a zinc-containing stereospecific methionine sulfoxide reductase. Proc. Natl. Acad / Sci 99 : 4245 ~ 4250
Kumar, R. A. / 2002 / Reaction mechanism, evolutionary analysis, and role of zinc in Drosophila methionine-R-sulfoxide reductase. J. Biol / Chem 277 : 37527 ~ 37535
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