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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제18권 제1호
- 발행연도
- 2008.1
- 수록면
- 5 - 14 (10page)
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초록· 키워드
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The deep subseafloor rock in oil reservoirs represents a
unique environment in which a high oil contamination
and a very low biomass can be observed. Sampling this
environment has been a challenge owing to the techniques
used for drilling and coring. In this study, the facilities
developed by the Brazilian oil company PETROBRAS for
accessing deep subsurface oil reservoirs were used to
obtain rock samples at 2,822-2,828 m below the ocean
floor surface from a virgin field located in the Atlantic
Ocean, Rio de Janeiro. To address the bacterial diversity
of these rock samples, PCR amplicons were obtained
using the DNA from four core sections and universal
primers for 16S rRNA and for APS reductase (aps) genes.
Clone libraries were generated from these PCR fragments
and 87 clones were sequenced. The phylogenetic analyses of
the 16S rDNA clone libraries showed a wide distribution
of types in the domain bacteria in the four core samples,
and the majority of the clones were identified as belonging to
Betaproteobacteria. The sulfate-reducing bacteria community
could only be amplified by PCR in one sample, and all clones
were identified as belonging to Gammaproteobacteria. For
the first time, the bacterial community was assessed in
such a deep subsurface environment.
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참고문헌 (23)
참고문헌 신청
Amann, R. I. / 1995 / Phylogenetic identification and in situ detection of individual microbial cells without cultivation. Microbiol / Rev 59 : 143 ~ 169
Andreoni, V. / 2004 / Bacterial communities and enzyme activities of PAHs polluted soils / Chemosphere 57 : 401 ~ 412
Barton, H. A. / 2006 / DNA extraction from low-biomass carbonate rock: An improved method with reduced contamination and the low-biomass contaminant database. J. Microbiol /
Boivin-Jahns, V. / 1996 / Bacterial diversity in a deep-subsurface clay environment. Appl. Enviro / Microbiol 62 : 3405 ~ 3412
Cunha, C. D. / 2006 / Oil biodegradation by Bacillus strains isolated from the rock of an oil reservoir located in a deepwater production basin in Brazil. Appl. Microbiol / B
Andreoni, V. / 2004 / Bacterial communities and enzyme activities of PAHs polluted soils / Chemosphere 57 : 401 ~ 412
Barton, H. A. / 2006 / DNA extraction from low-biomass carbonate rock: An improved method with reduced contamination and the low-biomass contaminant database. J. Microbiol /
Boivin-Jahns, V. / 1996 / Bacterial diversity in a deep-subsurface clay environment. Appl. Enviro / Microbiol 62 : 3405 ~ 3412
Cunha, C. D. / 2006 / Oil biodegradation by Bacillus strains isolated from the rock of an oil reservoir located in a deepwater production basin in Brazil. Appl. Microbiol / B
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