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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제19권 제12호
- 발행연도
- 2009.1
- 수록면
- 1,628 - 1,634 (7page)
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초록· 키워드
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Small heat shock proteins (sHSPs) function as molecular
chaperones that protect cells against environmental
stresses. In the present study, the genes of hsp17.6 and
hsp17.7, cytosolic class I sHSPs, were cloned from a
tropical plant, Ageratina adenophorum. Their C-terminal
domains were highly conserved with those of sHSPs from
other plants, indicating the importance of the C-terminal
domains for the structure and activity of sHSPs. The
recombinant HSP17.6 and HSP17.7 were applied to
determine their chaperone function. In vitro, HSP17.6 and
HSP17.7 actively participated in the refolding of the
model substrate citrate synthase (CS) and effectively
prevented the thermal aggregation of CS at 45oC and the
irreversible inactivation of CS at 38oC at stoichiometric
levels. The prior presence of HSP17.7 was assumed to
suppress the thermal aggregation of the model substrate
CS. Therefore, this report confirms the chaperone activity
of HSP17.6 and HSP17.7 and their potential as a
protectant for active proteins.
목차
등록된 정보가 없습니다.
참고문헌 (23)
참고문헌 신청
Ahrman, E. / 2007 / Chemical cross-linking of the chloroplast localized small heat-shock protein, Hsp21, and the model substrate citrate synthase / Protein Sci 16 : 1464 ~ 14
Ahrman, E. / 2007 / Small heat shock proteins prevent aggregation of citrate synthase and bind to the N-terminal region which is absent in thermostable forms of citrate synth
Basha, S. E. / 2006 / The Nterminal arm of small heat shock proteins is important for both chaperone activity and substrate specificity / J. Biol. Chem 281 : 39943 ~ 39952
Buncher, J. / 1996 / Supervising the fold: Functional principles of molecular chaperones / FASEB J 10 : 10 ~ 19
Collada, C. / 1997 / Purification and in vitro chaperone activity of a class I small heat-shock protein abundant in recalcitrant chestnut seeds / Plant Physiol 115 : 71 ~ 77
Ahrman, E. / 2007 / Small heat shock proteins prevent aggregation of citrate synthase and bind to the N-terminal region which is absent in thermostable forms of citrate synth
Basha, S. E. / 2006 / The Nterminal arm of small heat shock proteins is important for both chaperone activity and substrate specificity / J. Biol. Chem 281 : 39943 ~ 39952
Buncher, J. / 1996 / Supervising the fold: Functional principles of molecular chaperones / FASEB J 10 : 10 ~ 19
Collada, C. / 1997 / Purification and in vitro chaperone activity of a class I small heat-shock protein abundant in recalcitrant chestnut seeds / Plant Physiol 115 : 71 ~ 77
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