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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제20권 제7호
- 발행연도
- 2010.1
- 수록면
- 1,114 - 1,120 (7page)
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초록· 키워드
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A chitosan-degrading fungus, BSF114, was isolated from soil. The culture preparation showed strong chitosanolytic enzyme activity at an optimum pH of 4.0 and optimum temperature of 60oC after 36-40 h fermentation. The rapid decrease in the viscosity of the chitosan solution early in the reaction suggested an endo-type cleavage of the polymeric chitosan chains. To identify the isolated fungus, molecular biological and morphological methods were used. The fungal internal transcribed spacer (ITS)region 1 was amplified, sequenced, and then compared with related sequences in the GenBank database using BLAST.
The phylogenetic relationships were then analyzed, and the results showed that the fungus belongs to Aspergillus fumigatus. Morphological observations were also used to confirm the above conclusion. The chitooligosaccharides (COS) obtained through hydrolyzing the colloidal chitosan showed that A. fumigatus BSF114 is suitable for degrading chitosan and producing chitooligosaccharides on a large scale. High concentrations of the COS (1,000 and 500 μg/ml)significantly proliferated mice marrow cells.
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참고문헌 (22)
참고문헌 신청
Aktuganov, G. E / 2003 / Isolation and characterization of chitosanase from the strain Bacillus sp.739 / Appl. Biochem. Microb 39 : 469 ~ 474
Belalia, R / 2008 / New bioactive biomaterials based on quaternized chitosan / J. Agric. Food Chem 56 : 1582 ~ 1588
Bernhard, J / 1997 / Isolation and characterization of a pigmentless-conidium mutant of Aspergillus fumigatus with altered conidial surface and reduced virulence / Infect. Im
Cheng, C. Y / 2006 / Exploration of glycosyl hydrolase family 75, a chitosanase from Aspergillus fumigatus / J. Biol. Chem 6 : 3137 ~ 3144
Choi, Y. J / 2004 / Purification and characterization of chitosanase from Bacillus sp. strain kctc 0377bp and its application for the production of chitosan oligosaccharides
Belalia, R / 2008 / New bioactive biomaterials based on quaternized chitosan / J. Agric. Food Chem 56 : 1582 ~ 1588
Bernhard, J / 1997 / Isolation and characterization of a pigmentless-conidium mutant of Aspergillus fumigatus with altered conidial surface and reduced virulence / Infect. Im
Cheng, C. Y / 2006 / Exploration of glycosyl hydrolase family 75, a chitosanase from Aspergillus fumigatus / J. Biol. Chem 6 : 3137 ~ 3144
Choi, Y. J / 2004 / Purification and characterization of chitosanase from Bacillus sp. strain kctc 0377bp and its application for the production of chitosan oligosaccharides
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