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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제25권 제9호
- 발행연도
- 2015.1
- 수록면
- 1,417 - 1,424 (8page)
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In this study, we tried to characterize Streptomyces peucetius CYP157C4 with homology modeling using three cytochrome P450 (CYP) structures (CYP157C1, CYP164A2, and CYP107L1), having discovered that CYP157C4 lacks the ExxR motif that was considered invariant in all CYPs. We used Discovery Studio 3.5 to build our model after first assessing the stereochemical quality and side-chain environment, and a 7-ethoxycoumarin substrate was docked into the final model. The model-substrate complex allowed us to identify functionally important residues and validate the active-site architecture. We found a distance of 4.56 Å between the 7-ethoxycoumarin and the active site of the heme, and cloning and an in vitro assay of the CYP157C4 showed the dealkylation of the substrate. Since the details regarding this group of CYP structures are still unknown, the findings of this study may provide elucidation to assist with future efforts to find a legitimate substrate.
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참고문헌 (26)
참고문헌 신청
/ / Discovery Studio 3.5
Agnew CR / 2012 / An enlarged, adaptable active site in CYP164 family P450 enzymes, the sole P450 in Mycobacterium leprae / Antimicrob. Agents Chemother 56 : 391 ~ 402
Baudry J / 2006 / Class dependent sequence alignment strategy improves the structural and functional modeling of P450s / Protein Eng. Des. Selec 19 : 345 ~ 353
Bernhardt R. / 1996 / Cytochrome P450: structure, function, and generation of reactive oxygen species / Rev. Physiol. Biochem. Pharmacol 127 : 137 ~ 221
Bhattarai, Saurabh / 2012 / Homology Modeling and Docking Studies of Streptomyces peucetius CYP147F1 as Limonene Hydroxylase / Journal of Microbiology and Biotechnology 22 (7
Agnew CR / 2012 / An enlarged, adaptable active site in CYP164 family P450 enzymes, the sole P450 in Mycobacterium leprae / Antimicrob. Agents Chemother 56 : 391 ~ 402
Baudry J / 2006 / Class dependent sequence alignment strategy improves the structural and functional modeling of P450s / Protein Eng. Des. Selec 19 : 345 ~ 353
Bernhardt R. / 1996 / Cytochrome P450: structure, function, and generation of reactive oxygen species / Rev. Physiol. Biochem. Pharmacol 127 : 137 ~ 221
Bhattarai, Saurabh / 2012 / Homology Modeling and Docking Studies of Streptomyces peucetius CYP147F1 as Limonene Hydroxylase / Journal of Microbiology and Biotechnology 22 (7
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