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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
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- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제30권 제5호
- 발행연도
- 2020.1
- 수록면
- 777 - 784 (8page)
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Self-sufficient P450s, due to their fused nature, are the most effective tools for electron transfer to activate C-H bonds. They catalyze the oxygenation of fatty acids at different omega positions. Here, two new, self-sufficient cytochrome P450s, named ‘CYP102A15 and CYP102A170,’ from polar Bacillus sp. PAMC 25034 and Paenibacillus sp. PAMC 22724, respectively, were cloned and expressed in E. coli. The genes are homologues of CYP102A1 from Bacillus megaterium. They catalyzed the hydroxylation of both saturated and unsaturated fatty acids ranging in length from C12–C20, with a moderately diverse profile compared to other members of the CYP102A subfamily. CYP102A15 exhibited the highest activity toward linoleic acid with Km 15.3 μM, and CYP102A170 showed higher activity toward myristic acid with Km 17.4 μM. CYP10A170 also hydroxylated the Eicosapentaenoic acid at ω-1 position only. Various kinetic parameters of both monooxygenases were also determined.
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참고문헌 (37)
참고문헌 신청
Chowdhary PK / 2007 / Cloning, expression and characterization of a fast self-sufficient P450 : CYP102A5 from Bacillus cereus / Arch. Biochem. Biophys 468:32~43
Werck-Reichhart D / 2000 / Cytochromes P450 : a success story / Genome Biol :1
McLean KJ / 2005 / Biodiversity of cytochrome P450 redox systems / Biochem. Soc. Trans 33:796~801
Schmid A / 2002 / The use of enzymes in the chemical industry in Europe / Curr. Opin. Biotechnol 13:359~366
Urlacher VB / 2006 / Cytochrome P450 monooxygenases : perspectives for synthetic application / Trends Biotechnol 24:324~330
Werck-Reichhart D / 2000 / Cytochromes P450 : a success story / Genome Biol :1
McLean KJ / 2005 / Biodiversity of cytochrome P450 redox systems / Biochem. Soc. Trans 33:796~801
Schmid A / 2002 / The use of enzymes in the chemical industry in Europe / Curr. Opin. Biotechnol 13:359~366
Urlacher VB / 2006 / Cytochrome P450 monooxygenases : perspectives for synthetic application / Trends Biotechnol 24:324~330
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