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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제25권 제9호
- 발행연도
- 2015.1
- 수록면
- 1,410 - 1,416 (7page)
이용수
초록· 키워드
오류제보하기
Wolbachia is an obligate symbiotic bacteria that is ubiquitous in arthropods, with 25–70% of insect species estimated to be infected. Wolbachia species can interact with their insect hosts in a mutualistic or parasitic manner. Sequence types (ST) of Wolbachia are determined by multilocus sequence typing (MLST) of housekeeping genes. However, there are some limitations to MLST with respect to the generation of clone libraries and the Sanger sequencing method when a host is infected with multiple STs of Wolbachia. To assess the feasibility of massive parallel sequencing, also known as next-generation sequencing, we used pyrosequencing for sequence typing of Wolbachia in butterflies. We collected three species of butterflies (Eurema hecabe, Eurema laeta, and Tongeia fischeri) common to Korea and screened them for Wolbachia STs. We found that T. fischeri was infected with a single ST of Wolbachia, ST41. In contrast, E. hecabe and E. laeta were each infected with two STs of Wolbachia, ST41 and ST40. Our results clearly demonstrate that pyrosequencing-based MLST has a higher sensitivity than cloning and Sanger sequencing methods for the detection of minor alleles.
Considering the high prevalence of infection with multiple Wolbachia STs, next-generation sequencing with improved analysis would assist with scaling up approaches to Wolbachia MLST.
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등록된 정보가 없습니다.
참고문헌 (24)
참고문헌 신청
Atyame CM / 2011 / Diversification of Wolbachia endosymbiont in the Culex pipiens mosquito / Mol. Biol. Evol 28 : 2761 ~ 2772
Baldo L / 2006 / Multilocus sequence typing system for the endosymbiont Wolbachia pipientis / Appl. Environ. Microbiol 72 : 7098 ~ 7110
Bing XL / 2014 / Diversity and evolution of the Wolbachia endosymbionts of Bemisia (Hemiptera: Aleyrodidae) whiteflies / Ecol. Evol 4 : 2714 ~ 2737
Edgar RC / 2011 / UCHIME improves sensitivity and speed of chimera detection / Bioinformatics 27 : 2194 ~ 2200
Gilles A / 2011 / Accuracy and quality assessment of 454 GS-FLX titanium pyrosequencing / BMC Genomics 12 : 245 ~
Baldo L / 2006 / Multilocus sequence typing system for the endosymbiont Wolbachia pipientis / Appl. Environ. Microbiol 72 : 7098 ~ 7110
Bing XL / 2014 / Diversity and evolution of the Wolbachia endosymbionts of Bemisia (Hemiptera: Aleyrodidae) whiteflies / Ecol. Evol 4 : 2714 ~ 2737
Edgar RC / 2011 / UCHIME improves sensitivity and speed of chimera detection / Bioinformatics 27 : 2194 ~ 2200
Gilles A / 2011 / Accuracy and quality assessment of 454 GS-FLX titanium pyrosequencing / BMC Genomics 12 : 245 ~
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