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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제27권 제8호
- 발행연도
- 2017.1
- 수록면
- 1,428 - 1,440 (13page)
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초록· 키워드
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Phomopsis sp. XP-8 (an endophytic fungus) was previously found to produce pinoresinol diglucoside (PDG), a major antihypertensive compound of Tu-Chung (the bark of Eucommia ulmoides Oliv.), which is widely used in Chinese traditional medicines. In the present study, two bioconversion systems were developed for the production of PDG in Tris-HCl buffer containing glucose and Phomopsis sp. XP-8 cells (both resting and freeze-dried). When other factors remained unchanged, the bioconversion time, glucose concentration, cell ages, cell dosage, pH, temperature, and stirring speed influenced PDG production in a similar and decreasing manner after an initial increase with increasing levels for each factor. Considering the simultaneous change of various factors, the optimal conditions for PDG production were established as 70 g/l cells (8-day-old), 14 g/l glucose, 28°C, pH 7.5, and 180 rpm for systems employing resting cells, and 3.87 g/l cells, 14.67 g/l glucose, 28°C, pH 7.5, and 180 rpm for systems employing freeze-dried cells. The systems employing freeze-dried cells showed lower peak PDG production (110.28 μg/l), but at a much shorter time (12.65 h) compared with resting cells (23.62 mg/l, 91.5 h). The specific PDG production levels were 1.92 and 24 μg per gram cells per gram glucose for freeze-dried cells and resting cells, respectively. Both systems indicated a new and potentially efficient way to produce PDG independent of microbial cell growth.
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참고문헌 (22)
참고문헌 신청
Dong X / 2016 / Chemical constituents from Daphne giraldii Nitsche and their contents simultaneous determination by HPLC. Evid. Based Complement / Alternat. Med 2016 : 94923
Erwei Liu / 2012 / Eucommia ulmoides Bark Protects Against Renal Injury in Cadmium-Challenged Rats / Journal of Medicinal Food 15 (3) : 307 ~ 314
Hano C / 2006 / Pinoresinol?lariciresinol reductase gene expression and secoisolariciresinol diglucoside accumulation in developing flax (Linum usitatissimum) seeds / Planta
Hemmati S / 2007 / (+)-Pinoresinol/(-)-lariciresinol reductase from Linum perenne Himmelszelt involved in the biosynthesis of justicidin B / FEBS Lett 581 : 603 ~ 610
Huang RH / 2002 / Two novel antifungal peptides distinct with a fivedisulfide motif from the bark of Eucommia ulmoides Oliv / FEBS Lett 521 : 87 ~ 90
Erwei Liu / 2012 / Eucommia ulmoides Bark Protects Against Renal Injury in Cadmium-Challenged Rats / Journal of Medicinal Food 15 (3) : 307 ~ 314
Hano C / 2006 / Pinoresinol?lariciresinol reductase gene expression and secoisolariciresinol diglucoside accumulation in developing flax (Linum usitatissimum) seeds / Planta
Hemmati S / 2007 / (+)-Pinoresinol/(-)-lariciresinol reductase from Linum perenne Himmelszelt involved in the biosynthesis of justicidin B / FEBS Lett 581 : 603 ~ 610
Huang RH / 2002 / Two novel antifungal peptides distinct with a fivedisulfide motif from the bark of Eucommia ulmoides Oliv / FEBS Lett 521 : 87 ~ 90
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