지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2018.1
- 수록면
- 194 - 200 (7page)
이용수
초록· 키워드
오류제보하기
Glycogen plays important roles in bacteria. Its structure and storage capability have received more attention recently because of the potential correlations with environmental durability and pathogenicity. However, the low level of intracellular glycogen makes extraction and structure characterization difficult, inhibiting functional studies. Bacteria grown in regular media such as lysogeny broth and tryptic soy broth do no accumulate large amounts of glycogen. Comparative analyses of bacterial media reported in literature for glycogen-related studies revealed that there was no consistency in the recipes reported. Escherichia coli DH5α is a convenient model organism for gene manipulation studies with respect to glycogen. Additionally, M9 minimal salts medium is widely used to improve glycogen accumulation, although its composition varies. In this study, we optimized the M9 medium by adjusting the concentrations of itrogen source, tryptone, carbon source, and glucose, in order to achieve a balance between the growth rate and glycogen accumulation. Our result showed that 1 × M9 minimal salts medium containing 0.4% tryptone and 0.8% glucose was a well-balanced nutrient source for enhancing the growth and glycogen storage in bacteria. This result will help future investigations related to bacterial physiology in terms of glycogen function.
목차
등록된 정보가 없습니다.
참고문헌 (35)
참고문헌 신청
Wilson WA / 2010 / Regulation of glycogen metabolism in yeast and bacteria / FEMS Microbiol. Rev. 34:952~958
Wang L / 2011 / Glycogen with short average chain length enhances bacterial durability / Naturwissenschaften 98:719~729
Melendez R / 1997 / How did glycogen structure evolve to satisfy the requirement for rapid mobilization of glucose? A problem of physical constraints in structure building / J. Mol. Evol. 45:446~455
Chandra G / 2011 / Unexpected and widespread connections between bacterial glycogen and trehalose metabolism / Microbiology 157:1565~1572
Bourassa L / 2009 / Glycogen contributes to the environmental persistence and transmission of Vibrio cholerae / Mol. Microbiol. 72:124~138
Wang L / 2011 / Glycogen with short average chain length enhances bacterial durability / Naturwissenschaften 98:719~729
Melendez R / 1997 / How did glycogen structure evolve to satisfy the requirement for rapid mobilization of glucose? A problem of physical constraints in structure building / J. Mol. Evol. 45:446~455
Chandra G / 2011 / Unexpected and widespread connections between bacterial glycogen and trehalose metabolism / Microbiology 157:1565~1572
Bourassa L / 2009 / Glycogen contributes to the environmental persistence and transmission of Vibrio cholerae / Mol. Microbiol. 72:124~138
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Glycogen Metabolism in Vibrio vulnificus Affected by malP and malQ
한국미생물·생명공학회지
2018 .01
-
Glycogen as a Regulator of White Fat Browning
Molecules and Cells
2022 .04
-
A CoMFA Study of Glycogen Synthase Kinase 3 Inhibitors
조선자연과학논문집
2015 .01
-
Crystal Structure of Glycogen Branching Enzyme from Pyrococcus horikoshii
한국미생물학회 학술대회논문집
2016 .04
-
Separation efficiency of glycogen and pullulan in asymmetric flow field-flow fractionation: Difference between conventional focusing and hydrodynamic-relaxation frit inlet
한국분석과학회 학술대회
2018 .11
-
Characterization of the Transglycosylation Reaction of 4-α- Glucanotransferase (MalQ) and Its Role in Glycogen Breakdown in Escherichia coli
Journal of Microbiology and Biotechnology
2019 .01
-
Increased glucose metabolism and alpha-glucosidase inhibition in Cordyceps militaris water extract-treated HepG2 cells
Nutrition Research and Practice
2017 .06
-
Increased glucose metabolism and alpha-glucosidase inhibition in Cordyceps militaris water extract-treated HepG2 cells
Nutrition Research and Practice
2017 .06
-
Design, Synthesis and Evaluation of Pentacyclic Triterpenoids Similar to Glycyrrhetinic Acid Via Combination of Chemical and Microbial Modification as Glycogen Phosphorylases Inhibitor
Journal of Microbiology and Biotechnology
2018 .01
-
Evolution of Escherichia coli for Optimal Growth in a Rich Medium
한국미생물학회 학술대회논문집
2019 .04
-
Recombinant Human Laforin Expressed in Insect Cells: Expression, Purification, and Biochemical Characterizations
대한화학회지
2015 .10
-
Statistical Optimization of Medium Components by Response Surface Methodology to Enhance Menaquinone-7 (Vitamin K2) Production by Bacillus subtilis
Journal of Microbiology and Biotechnology
2018 .01
-
Anti-fatigue effect of tormentic acid through alleviating oxidative stress and energy metabolism-modulating property in C2C12 cells and animal models
Nutrition Research and Practice
2023 .08
-
Anti-fatigue effect of tormentic acid through alleviating oxidative stress and energy metabolism-modulating property in C2C12 cells and animal models
Nutrition Research and Practice
2023 .08
-
합성 유전자를 이용하여 Escherichia coli에서 백신 후보의 생산 혹은 진단용 항체의 개발을 위한 인간 rotavirus VP8* 부분 단백질의 발현
생명과학회지
2018 .04
-
Gynostemma pentaphyllum extract and its active component gypenoside L improve the exercise performance of treadmill-trained mice
Nutrition Research and Practice
2022 .06
-
Gynostemma pentaphyllum extract and its active component gypenoside L improve the exercise performance of treadmill-trained mice
Nutrition Research and Practice
2022 .06
-
Highly Efficient Biotransformation of Notoginsenoside R1 into Ginsenoside Rg1 by Dictyoglomus thermophilum β -xylosidase Xln-DT
Journal of Microbiology and Biotechnology
2022 .04
-
A Novel PHKA1 Mutation in a Patient with Glycogen Storage Disease Type IXD
생명과학회지
2020 .08
-
세균 배양액과 반응한 Lactobacillus plantarum의 생장속도 및 항균 활성에 대한 연구
한국미생물·생명공학회지
2015 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0