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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제20권 제8호
- 발행연도
- 2010.1
- 수록면
- 1,210 - 1,214 (5page)
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초록· 키워드
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Many bacteria are involved in the fermentation of doenjang,and Bacillus species are known to perform significant roles. Although SDS-PAGE has been frequently used to classify and identify bacteria in various samples, the microbial diversity in doenjang has not yet been investigated.
This study aims to determine the identity and distribution of dominant Bacillus species in doenjang using SDSPAGE profiles of whole-cell proteins and 16S rRNA gene sequencing. Reference Bacillus strains yielded differential SDS-PAGE banding patterns that could be considered to be highly specific fingerprints. Grouping of bacterial strains isolated from doenjang samples by whole-cell protein patterns was confirmed by analysis of their 16S rRNA gene sequences. B. subtilis was found to be the most dominant strain in most of the samples, whereas B. licheniformis and B. amyloliquefaciens were less frequently found but were also detected in several samples. The results obtained in this study show that a combined identification method using SDS-PAGE profiles of whole-cell proteins and subsequent 16S rRNA gene sequence analysis could successfully identify Bacillus species isolated from doenjang.
목차
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참고문헌 (18)
참고문헌 신청
CHUNG, KUN SUB / 2006 / Isolation of Bacteria Producing a B-Cell-Specific Biological Response Modifier Found in Korean Fermented Soybean Paste / Journal of Microbiology and B
Choi, M. R / 1999 / Selective cytotoxic effects of doenjang (Korean soybean paste) fermented with Bacillus strains on human liver cell lines / J. Microbiol. Biotechnol 9 : 50
Dicks, L. M. T / 1990 / Taxonomy of Leuconostoc species, particularly Leuconostoc oenos, as revealed by numerical analysis of total soluble cell protein patterns, DNA base co
Ji, W. D / 1995 / Volatile components of Korean soybean paste produced by Bacillus subtilis PM3 / J. Microbiol. Biotechnol 5 : 143 ~ 148
Jin, Jianbo / 2008 / Diversity Analysis of Lactic Acid Bacteria in Takju, Korean Rice Wine / Journal of Microbiology and Biotechnology 18 (10) : 1678 ~ 1682
Choi, M. R / 1999 / Selective cytotoxic effects of doenjang (Korean soybean paste) fermented with Bacillus strains on human liver cell lines / J. Microbiol. Biotechnol 9 : 50
Dicks, L. M. T / 1990 / Taxonomy of Leuconostoc species, particularly Leuconostoc oenos, as revealed by numerical analysis of total soluble cell protein patterns, DNA base co
Ji, W. D / 1995 / Volatile components of Korean soybean paste produced by Bacillus subtilis PM3 / J. Microbiol. Biotechnol 5 : 143 ~ 148
Jin, Jianbo / 2008 / Diversity Analysis of Lactic Acid Bacteria in Takju, Korean Rice Wine / Journal of Microbiology and Biotechnology 18 (10) : 1678 ~ 1682
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