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논문 기본 정보
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- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제19권 제1호
- 발행연도
- 2009.1
- 수록면
- 72 - 77 (6page)
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초록· 키워드
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Here, we demonstrate that the overexpression of the
GroELS chaperone system, which assists the folding of
intracellular proteins and prevents aggregation of its
biological targets, can enhance the thermotolerance of
Escherichia coli strains and facilitate the production of
recombinant protein under thermal stress. The overexpression
of GroELS led to an about 2-fold higher growth rate of E.
coli XL-1 blue than control at 45oC and induced the
growth of the strain even at 50oC, although the growth
was not sustained in the second-round culture. The effect
of GroELS overexpression was also effective on other E.
coli strains such as JM109, DH5α, and BL21. Finally, we
have shown that coexpression of GroELS allows us to
produce recombinant protein even at 50oC, a temperature
at which the protein production based on E. coli is not
efficient. This study indicates that the employment of the
GroELS overexpression system can expand the range of
environmental conditions for E. coli.
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참고문헌 (23)
참고문헌 신청
5. Chow, K. C. / 1998 / Overexpression of dnak/ dnaJ and GroEL confers freeze tolerance to E. coli / Biochem. Biophys. Res. Commun 253 : 502 ~ 505
Austain, B. / 1988 / Methods in Aquatic Bacteriology / Wiley-Interscience : 222 ~ 231
Baneyx, F. / 2004 / Recombinant protein folding and misfolding in Escherichia coli / Nat. Biotechnol 22 : 1399 ~ 1408
Betiku, E. / 2006 / Molecular chaperones involved in heterologous protein folding in Escherichia coli / Biotech. Mol. Biol. Rev 1 : 66 ~ 75
Bulter, T. / 2004 / Design of artificial cell-cell communication using gene and metabolic network / Proc. Natl. Acad. Sci. U.S.A. 101 : 2299 ~ 2304
Austain, B. / 1988 / Methods in Aquatic Bacteriology / Wiley-Interscience : 222 ~ 231
Baneyx, F. / 2004 / Recombinant protein folding and misfolding in Escherichia coli / Nat. Biotechnol 22 : 1399 ~ 1408
Betiku, E. / 2006 / Molecular chaperones involved in heterologous protein folding in Escherichia coli / Biotech. Mol. Biol. Rev 1 : 66 ~ 75
Bulter, T. / 2004 / Design of artificial cell-cell communication using gene and metabolic network / Proc. Natl. Acad. Sci. U.S.A. 101 : 2299 ~ 2304
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