지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
The synergistic effect of lowered incubation temperature and GroEL/ES expression on the production of soluble form of B. macerans cyclodextrin glucanotransferase (CGTase) was studied in recombinant E. coli. pTCGT1 and pGro11 carrying the cgt and groEL/ES genes under the control of T7 promoter and pzt-1 promoter, respectively, were co-introduced. Tetracycline (10 ng/ml) and IPTG (1 mM) were added at the early-exponential phase (2 hr) and mid-exponential phase (3 hr). Low temperature cultivation at 25℃ with groEL/ES expression improved the activity of CGTase by two fold, compared to 37℃ cultivation without chaperones. SDS-PAGE analysis revealed that about 69% of CGTase in the total CGTase protein was found in the soluble fraction by overexpression of GroEL/ES and cultivation at 25℃, whereas 20% of CGTase was detected in the soluble fraction when E. coli was cultivated at 37℃ without chaperone. The amount of soluble CGTase from 25℃ culture with chaperone was 3.5-fold higher than that of 37℃ culture without chaperone. Therefore the expression of GroEL/ES and low temperature cultivation greatly enhanced the soluble production of CGTase in E. coli.
목차
재료 및 방법
결과 및 고찰
요 약
감사의 글
참 고 문 헌
참고문헌 (26)
참고문헌 신청
[학술지(정기간행물)] - / 1993 / Inclusion body formation and protein stability in sequence variants of interleukin-1 : 18053 ~ 18061
[학술지(정기간행물)] - / 2001 / Chaperone- assisted overexpression of an active D-carbamoylase from Agrobacterium tumefaciens AM10 23 : 374 ~ 379
[학술지(정기간행물)] - / 1992 / Cooperation of GroEL/GroES and DnaK/DnaJ heat shock proteins in preventing protein misfolding in Escherichia coli : 10341 ~ 10344
[학술지(정기간행물)] - / 1999 / Enhancement of solubility of Bacillus macerans cyclodextrin glucanotransferase by thioredoxin fusion 8 : 276 ~ 279
[학술지(정기간행물)] - / 2001 / Effects of environmental factors on in vivo folding of Bacillus macerans cyclodextrin glycosyltransferase in recombinant Escherichia coli 11 : 92 ~ 96
[학술지(정기간행물)] - / 2001 / Chaperone- assisted overexpression of an active D-carbamoylase from Agrobacterium tumefaciens AM10 23 : 374 ~ 379
[학술지(정기간행물)] - / 1992 / Cooperation of GroEL/GroES and DnaK/DnaJ heat shock proteins in preventing protein misfolding in Escherichia coli : 10341 ~ 10344
[학술지(정기간행물)] - / 1999 / Enhancement of solubility of Bacillus macerans cyclodextrin glucanotransferase by thioredoxin fusion 8 : 276 ~ 279
[학술지(정기간행물)] - / 2001 / Effects of environmental factors on in vivo folding of Bacillus macerans cyclodextrin glycosyltransferase in recombinant Escherichia coli 11 : 92 ~ 96
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
E. coli에서 GroEL/ES chaperone 공발현에 의한 활성형 cyclodextrin glucanotransferase의 생산 증대
생명과학회지
2002 .12
-
GroEL/ES Chaperone and Low Culture Temperature Synergistically Enhancedthe Soluble Expression of CGTase in E. coli
Journal of Microbiology and Biotechnology
2004 .01
-
고온성이며 호알칼리성인 Bacillus cereus B-13으로부터 내열성, 호알카리성 Cyclodextrin Glucanotransferase의 생산과 특성
자연 과학 논문집 : 배재대학 첨단과학연구소
2005 .01
-
Bacillus subtilis에서 Bacillus stearothermophilus CGTase의 구성적 발현
생명과학회지
2004 .06
-
Saccharomyces cerevisiae에서 Bacillus CGTase의 표층발현
생명과학회지
2005 .02
-
Paenibacillus sp. JB-13 CGTase 유전자의 E. coli에서의 발현 및 효소 특성에 관한 연구
한국생명과학회 심포지움
2003 .05
-
Improving the Productivity of Recombinant Protein in Escherichia coli Under Thermal Stress by Coexpressing GroELS Chaperone System
Journal of Microbiology and Biotechnology
2009 .01
-
Paenibacillus sp. JB-13 Cyclodextrin Glucanotransferase 유전자의 E. coli에서의 발현 및 최적 생산
미생물학회지
2008 .03
-
Optimization of Cyclodextrin Glucanotransferase Immobilization on Amberlite IRA-900
생명과학회지
2004 .10
-
Continuous Synthesis of Escherichia coli GroEL at a High Temperature
The Journal of Microbiology
2000 .09
-
Production of Soluble Human Granulocyte Colony Stimulating Factor inE. coli by Molecular Chaperones
Journal of Microbiology and Biotechnology
2005 .01
-
세포내인자로서의 정교한 기능을 하는 molecular chaperone ( Molecular chaperone as a sophisticated intracellular membership )
생명과학회지
1998 .04
-
Effect of temperature and denaturation conditions on protein folding assisted by GroEL-GroES chaperonin
생명과학회지
2007 .02
-
폐렴구균 열충격 단백질 GroEL의 특성
미생물학회지
1998 .06
-
Effects of Chaperones on mRNA Stability and Gene Expression in Escherichia coli
Journal of Microbiology and Biotechnology
2008 .01
-
Enhancement of β-cyclodextrin Production and Fabrication of Edible Antimicrobial Films Incorporated with Clove Essential Oil/β-cyclodextrin Inclusion Complex
한국미생물·생명공학회지
2020 .01
-
대장균에서 분자 chaperone에 의한 alginate lyase의 가용성 발현 증대
생명과학회지
2007 .01
-
Soluble Expression of the Fucosyltransferase Gene from Helicobacter pylori in Escherichia coli by Co-expression of Molecular Chaperones
한국미생물·생명공학회지
2015 .01
-
Rescue of Oxidative Stress by Molecular Chaperones in Yeast
한국미생물학회 학술대회논문집
2001 .10
-
Escherichia coli GroEL was Induced by the Expression of the Cloned Bacillus megaterium ATCC14945 Penicillin G Acylase Gene
미생물학회지
1992 .12
이 논문의 저자 정보
이 논문과 함께 이용한 논문
최근 본 자료
댓글(0)
0
UCI(KEPA) : I410-ECN-0101-2009-470-014221320