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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제25권 제11호
- 발행연도
- 2015.1
- 수록면
- 1,936 - 1,943 (8page)
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초록· 키워드
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The present study developed an efficient and environmentally friendly method for recovering polyhydroxybutyrate (PHB) from Cupriavidus necator. Several non-halogenated solvents were tested and it was found that butyl acetate and ethyl acetate are powerful solvents for the biopolymer. Testing was performed to examine the effects of temperature (25°C until temperature below solvent boiling points) and heating incubation time (0-60 min) on the two solvents. Butyl acetate had a higher recovery level (96%) and product purity (up to 99%) than ethyl acetate at 103°C and a heating incubation time of 30 min. Under these conditions, PHB recorded the highest molecular weight of 1.4 × 106 compared with the standard procedure (i.e., recovery using chloroform). The proposed strategy showed that butyl acetate is a good alternative to halogenated solvents such as chloroform for recovery of PHB.
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참고문헌 (25)
참고문헌 신청
[
Akita S / 1976 / Solution properties of poly(D-β-hydroxybutyrate). 1. Biosynthesis and characterization / Macromolecules 9 : 774 ~ 780
Anderson-Cook CM / 2009 / Response surface design evaluation and comparison / J. Stat. Plan. Inference 139 : 629 ~ 641
Anis SN / 2013 / Increased recovery and improved purity of PHA from recombinant Cupriavidus necator / Bioengineered 4 : 115 ~ 118
Aramvash A / 2015 / Statistical physical and nutrient optimization of bioplastic polyhydroxybutyrate production by Cupriavidus necator / Int. J. Environ. Sci. Technol 12 : 23
Akita S / 1976 / Solution properties of poly(D-β-hydroxybutyrate). 1. Biosynthesis and characterization / Macromolecules 9 : 774 ~ 780
Anderson-Cook CM / 2009 / Response surface design evaluation and comparison / J. Stat. Plan. Inference 139 : 629 ~ 641
Anis SN / 2013 / Increased recovery and improved purity of PHA from recombinant Cupriavidus necator / Bioengineered 4 : 115 ~ 118
Aramvash A / 2015 / Statistical physical and nutrient optimization of bioplastic polyhydroxybutyrate production by Cupriavidus necator / Int. J. Environ. Sci. Technol 12 : 23
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