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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제26권 제3호
- 발행연도
- 2016.1
- 수록면
- 452 - 460 (9page)
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초록· 키워드
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Acid stress can affect the viability of probiotics, especially Bifidobacterium. This study aimed to improve the acid tolerance of Bifidobacterium longum BBMN68 using adaptive evolution. The stress response, and genomic differences of the parental strain and the variant strain were compared by acid stress. The highest acid-resistant mutant strain (BBMN68m) was isolated from more than 100 asexual lines, which were adaptive to the acid stress for 10th, 20th, 30th, 40th, and 50th repeats, respectively. The variant strain showed a significant increase in acid tolerance under conditions of pH 2.5 for 2 h (from 7.92 to 4.44 log CFU/ml) compared with the wildtype strain (WT, from 7.87 to 0 log CFU/ml). The surface of the variant strain was also smoother. Comparative whole-genome analysis showed that the galactosyl transferase D gene (cpsD, bbmn68_1012), a key gene involved in exopolysaccharide (EPS) synthesis, was altered by two nucleotides in the mutant, causing alteration in amino acids, pI (from 8.94 to 9.19), and predicted protein structure. Meanwhile, cpsD expression and EPS production were also reduced in the variant strain (p < 0.05) compared with WT, and the exogenous WT-EPS in the variant strain reduced its acid-resistant ability. These results suggested EPS was related to acid responses of BBMN68.
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참고문헌 (35)
참고문헌 신청
Alp G / 2010 / Relationship between the resistance to bile salts and low pH with exopolysaccharide (EPS)production of Bifidobacterium spp. isolated from infants feces and breast milk / Anaerobe 16:101~105
Brennan TCR / 2015 / Evolutionary engineering improves tolerance for replacement jet fuels in Saccharomyces cerevisiae / Appl. Environ. Microbiol 81:3316~3325
Cesena C / 2001 / Lactobacillus crispatus and its nonaggregating mutant in human colonization trials / J. Dairy Sci 84:1001~1010
Colvin KM / 2011 / The Pel polysaccharide can serve a structural and protective role in the biofilm matrix of Pseudomonas aeruginosa / PloS Pathog 7:e1001264
Colvin KM / 2012 / The Pel and Psl polysaccharides provide Pseudomonas aeruginosa structural redundancy within the biofilm matrix / Environ. Microbiol 14:1913~1928
Brennan TCR / 2015 / Evolutionary engineering improves tolerance for replacement jet fuels in Saccharomyces cerevisiae / Appl. Environ. Microbiol 81:3316~3325
Cesena C / 2001 / Lactobacillus crispatus and its nonaggregating mutant in human colonization trials / J. Dairy Sci 84:1001~1010
Colvin KM / 2011 / The Pel polysaccharide can serve a structural and protective role in the biofilm matrix of Pseudomonas aeruginosa / PloS Pathog 7:e1001264
Colvin KM / 2012 / The Pel and Psl polysaccharides provide Pseudomonas aeruginosa structural redundancy within the biofilm matrix / Environ. Microbiol 14:1913~1928
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