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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제26권 제11호
- 발행연도
- 2016.1
- 수록면
- 1,918 - 1,923 (6page)
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초록· 키워드
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Two baicalein derivatives, baicalin and oroxylin A, were synthesized in this study. These derivatives exhibit diverse biological activities, such as anxiolytic and anticancer activities as well as memory enhancement. In order to synthesize baicalin from aglycon baicalein using Escherichia coli, we utilized a glycosyltransferase that regioselectively transfers glucuronic acid from UDP-glucuronic acid to the 7-hydroxy group of baicalein. To increase baicalin productivity, an araA deletion E. coli mutant, which accumulates UDP-glucuronic acid, was used, and ugd, which converts UDP-glucose to UDP-glucuronic acid, was overexpressed. Using these strategies, approximately 720.3 μM baicalin was synthesized from 1,000 μM baicalein. Oroxylin A was then synthesized from baicalein. Two O-methyltransferases (OMTs), ROMT-15 and POMT-9, were tested to examine the production of oroxylin A from baicalein. E. coli harboring ROMT-15 and E. coli harboring POMT-9 produced reaction products that had different retention times, indicating that they are methylated at different positions; the structure of the reaction product from POMT-9 was consistent with oroxylin A, whereas that from ROMT-15 was 7-O-methyl baicalein. Using E. coli harboring POMT-9, approximately 50.3 mg/l of oroxylin A (177 μM) was synthesized from 54 mg/l baicalein (200 μM).
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참고문헌 (24)
참고문헌 신청
An DG / 2016 / Biosynthesis of two quercetin O-diglycosides in Escherichia coli / J. Ind. Microbiol. Biotechnol 43:841~849
Breazeale SD / 2005 / A formyltransferase required for polymyxin resistance in Escherichia coli and the modification of lipid A with 4-amino-4-deoxy-L-arabinose / J. Biol. Chem 280:14154~14167
Chen YC / 2000 / Oroxylin A inhibition of lipopolysaccharide-induced iNOS and COX-2 gene expression via suppression of nuclear factor-kappaB activation / Biochem. Pharmacol 59:1445~1457
Díaz E / 2001 / Biodegradation of aromatic compounds by Escherichia coli / Microbiol. Mol. Biol. Rev 65:523~569
Gao J / 2008 / Validation of a HPLC method for flavonoid biomarkers in skullcap (Scutellaria) and its use to illustrate wide variability in the quality of commercial tinctures / J. Pharm. Pharm. Sci 11:77~87
Breazeale SD / 2005 / A formyltransferase required for polymyxin resistance in Escherichia coli and the modification of lipid A with 4-amino-4-deoxy-L-arabinose / J. Biol. Chem 280:14154~14167
Chen YC / 2000 / Oroxylin A inhibition of lipopolysaccharide-induced iNOS and COX-2 gene expression via suppression of nuclear factor-kappaB activation / Biochem. Pharmacol 59:1445~1457
Díaz E / 2001 / Biodegradation of aromatic compounds by Escherichia coli / Microbiol. Mol. Biol. Rev 65:523~569
Gao J / 2008 / Validation of a HPLC method for flavonoid biomarkers in skullcap (Scutellaria) and its use to illustrate wide variability in the quality of commercial tinctures / J. Pharm. Pharm. Sci 11:77~87
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