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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제28권 제11호
- 발행연도
- 2018.1
- 수록면
- 1,782 - 1,790 (9page)
이용수
초록· 키워드
오류제보하기
Assessment of microorganism viability is useful in many industrial fields. A large number of methods associated with the use of fluorescent probes have been developed, including fluorimetry, fluorescence microscopy, and cytometry. In this study, a microvolume spectrofluorometer was used to measure the membrane potential variations of Escherichia coli. In order to estimate the sensitivity of the device, the membrane potential of E. coli was artificially disrupted using an ionophore agent: carbonyl cyanide 3-chlorophenylhydrazone. The membrane potential was evaluated using two ratiometric methods: a Rhodamine 123/ 4’,6-diamidino-2-phenylindole combination and a JC-10 ratiometric probe. These methods were used to study the impact of freezing on E. coli, and were compared with the conventional enumeration method. The results showed that it was beneficial to use this compact, easy-touse, and inexpensive spectrofluorometer to assess the viability of bacterial cells via their membrane potential.
#Bacterial viability
#Escherichia coli
#fluorescence techniques
#membrane potentialBacterial viability
#Escherichia coli
#fluorescence techniques
#membrane potentialBacterial viability
#Escherichia coli
#fluorescence techniques
#membrane potentialBacterial viability
#Escherichia coli
#fluorescence techniques
#membrane potentialBacterial viability
#Escherichia coli
#fluorescence techniques
#membrane potentialBacterial viability
#Escherichia coli
#fluorescence techniques
#membrane potentialBacterial viability
#Escherichia coli
#fluorescence techniques
#membrane potential
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참고문헌 (40)
참고문헌 신청
Joux F / 2000 / Use of fluorescent probes to assess physiological functions of bacteria at single-cell level / Microbes Infect 2:1523~1535
Xu L / 2017 / Mechanisms of ultraviolet disinfection and chlorination of Escherichia coli:culturability, membrane permeability, metabolism, and genetic damage / J. Environ. Sci. (China) 65:356~366
Strahl H / 2010 / Membrane potential is important for bacterial cell division / Proc. Natl. Acad. Sci. USA 107:12281~12286
McFeters GA / 1995 / Physiological assessment of bacteria using fluorochromes / J. Microbiol. Methods 21:1~13
Breeuwer P / 2000 / Assessment of viability of microorganisms employing fluorescence techniques / Int. J. Food Microbiol 55:193~200
Xu L / 2017 / Mechanisms of ultraviolet disinfection and chlorination of Escherichia coli:culturability, membrane permeability, metabolism, and genetic damage / J. Environ. Sci. (China) 65:356~366
Strahl H / 2010 / Membrane potential is important for bacterial cell division / Proc. Natl. Acad. Sci. USA 107:12281~12286
McFeters GA / 1995 / Physiological assessment of bacteria using fluorochromes / J. Microbiol. Methods 21:1~13
Breeuwer P / 2000 / Assessment of viability of microorganisms employing fluorescence techniques / Int. J. Food Microbiol 55:193~200
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