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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제20권 제6호
- 발행연도
- 2010.1
- 수록면
- 968 - 973 (6page)
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초록· 키워드
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In order to study its biochemical properties, the integrase (IN) protein of feline foamy virus (FFV) was overexpressed in Escherichia coli, purified by two-step chromatography,(Talon column and heparin column), and characterized in biochemical aspects. For the three enzymatic reactions of the 3'-processing, strand transfer, and disintegration activities, the Mn2+ ion was essentially required as a cofactor. Interestingly, Co2+ and Zn2+ ions were found to act as effective cofactors, whereas other transition elements such as Ni2+, Cu2+, La3+, Y3+, Cd2+, Li1+, Ba2+, Sr2+, and V3+were not. Regarding the substrate specificity, FFV IN has low substrate specificities as it cleaved in a significant level prototype foamy virus (PFV) U5 LTR substrate as well as FFV U5 LTR substrate, whereas PFV IN did not.
Finally, the 3'-processing activity was observed in high concentrations of several solvents such as CHAPS, glycerol,Tween 20, and Triton X-100, which are generally used for dissolution of chemicals in inhibitor screening. Therefore,in this first report showing its biochemical properties,FFV IN is proposed to have low specificities on the use of cofactor and substrate for enzymatic reaction as compared with other retroviral INs.
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참고문헌 (23)
참고문헌 신청
Achong, B. G / 1971 / An unusual virus in cultures from a human nasopharyngeal carcinoma / J. Natl. Cancer Inst 46 : 299 ~ 307
Appa, R. S / 2001 / Role of the nonspecific DNA-binding region and α helices within the core domain of retroviral integrase in selecting target DNA sites for integration / J
Bujacz, G / 1997 / Binding of different divalent cations to the active site of avian sarcoma virus integrase and their effects on enzymatic activity / J. Biol. Chem 272 : 181
Bushman, F. D / 1990 / Sequence requirements for integration of Moloney murine leukemia virus DNA in vitro / J. Virol 64 : 5645 ~ 5648
Chaga, G / 1999 / Immobilized metal ion affinity chromatography on Co2+-carboxymethyl aspartate-agarose Superflow, as demonstrated by one-step purification of lactate dehydro
Appa, R. S / 2001 / Role of the nonspecific DNA-binding region and α helices within the core domain of retroviral integrase in selecting target DNA sites for integration / J
Bujacz, G / 1997 / Binding of different divalent cations to the active site of avian sarcoma virus integrase and their effects on enzymatic activity / J. Biol. Chem 272 : 181
Bushman, F. D / 1990 / Sequence requirements for integration of Moloney murine leukemia virus DNA in vitro / J. Virol 64 : 5645 ~ 5648
Chaga, G / 1999 / Immobilized metal ion affinity chromatography on Co2+-carboxymethyl aspartate-agarose Superflow, as demonstrated by one-step purification of lactate dehydro
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