지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제25권 제8호
- 발행연도
- 2015.1
- 수록면
- 1,307 - 1,314 (8page)
이용수
초록· 키워드
오류제보하기
Leukemia inhibitory factor (LIF) is a member of the IL-6 cytokine family, having pleiotropic actions such as maintaining stem cell pluripotency and enabling blastocyst implantation. Because the action of LIF is mediated by a ligand-receptor interaction with the LIF receptor (LIF-R), an antagonist for LIF-R has been developed to inhibit LIF-induced signaling. In this study, we present a novel method for the production and purification of an antagonist to human LIF-R (hLA). His-tagged hLA was expressed in E. coli, and simple purification methods without any endopeptidase cleavage were designed. In addition, we determined the optimal temperature conditions for enhancing the production of soluble hLA. Finally, the bioactivity of His-tagged hLA was examined using STAT3 phosphorylation and receptivity of human endometrial ECC-1 cells. Our strategy provides a rapid and efficient method to produce biologically active recombinant hLA.
목차
등록된 정보가 없습니다.
참고문헌 (24)
참고문헌 신청
Aschenbach LC / 2013 / The LIF receptor antagonist PEGLA is effectively delivered to the uterine endometrium and blocks LIF activity in cynomolgus monkeys / Contraception 87
Berkmen M. / 2012 / Production of disulfide-bonded proteins in Escherichia coli / Protein Expr. Purif 82 : 240 ~ 251
Ceroni A / 2006 / DISULFIND:a disulfide bonding state and cysteine connectivity prediction server / Nucleic Acids Res 34 : W177 ~ W181
Chen JR / 2000 / Leukemia inhibitory factor can substitute for nidatory estrogen and is essential to inducing a receptive uterus for implantation but is not essential for sub
Fairlie WD / 2004 / Affinity maturation of leukemia inhibitory factor and conversion to potent antagonists of signaling / J. Biol. Chem 279 : 2125 ~ 2134
Berkmen M. / 2012 / Production of disulfide-bonded proteins in Escherichia coli / Protein Expr. Purif 82 : 240 ~ 251
Ceroni A / 2006 / DISULFIND:a disulfide bonding state and cysteine connectivity prediction server / Nucleic Acids Res 34 : W177 ~ W181
Chen JR / 2000 / Leukemia inhibitory factor can substitute for nidatory estrogen and is essential to inducing a receptive uterus for implantation but is not essential for sub
Fairlie WD / 2004 / Affinity maturation of leukemia inhibitory factor and conversion to potent antagonists of signaling / J. Biol. Chem 279 : 2125 ~ 2134
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Lithium Fluoride(LiF)의 두께 변화가 유기 발광 다이오드의 특성에 미치는 영향
한국진공학회 학술발표회초록집
2018 .02
-
Improvement of the luminous efficiency of organic light emitting diode using LiF anode buffer layer
한국진공학회 학술발표회초록집
2015 .08
-
Efficient Hole Injection Control Using LiF Layer in Organic Light Emitting Diodes
한국진공학회 학술발표회초록집
2015 .02
-
Enhancement of charge injection by introducing LiF as anode buffer layer in hosphorescence Organic Light-Emitting Diodes
한국진공학회 학술발표회초록집
2018 .02
-
Targeted capture enrichment and sequencing identifies HLA variants associated with the severity of COVID-19
Genes & Genomics
2023 .04
-
Benzoic Acid Enhances Embryo Implantation through LIF-Dependent Expression of Integrin αVβ3 and αVβ5
Journal of Microbiology and Biotechnology
2017 .01
-
한국인 790명을 대상으로 한 HLA-A 및 HLA-DR 대립유전자 빈도 및 일배체형 연관성에 대한 종합적 분석
대한임상검사과학회지
2024 .09
-
Thickness Variation of Electron Injection Layer of Organic Light Emitting Diodes
한국진공학회 학술발표회초록집
2018 .08
-
Insignificant effects of loss of heterozygosity in HLA in the efficacy of immune checkpoint blockade treatment
Genes & Genomics
2022 .04
-
Association of HLA Genotype and Fulminant Type 1 Diabetes in Koreans
Genomics & informatics
2015 .01
-
Bavachin Suppresses Alpha-Hemolysin Expression and Protects Mice from Pneumonia Infection by Staphylococcus aureus
Journal of Microbiology and Biotechnology
2022 .10
-
Low Melting-point Diffusion Salts of Heavy Rare-earth for Enhancing Coercivity of Grain-boundary-diffusion-processed Nd-Fe-B-type Magnet
Journal of Magnetics
2020 .06
-
Effects of Co-Expression of Liver X Receptor β-Ligand Binding Domain with its Partner, Retinoid X Receptor α-Ligand Binding Domain, on their Solubility and Biological Activity in Escherichia coli
Journal of Microbiology and Biotechnology
2015 .01
-
Isorhamnetin Attenuates Staphylococcus aureus-Induced Lung Cell Injury by Inhibiting Alpha-Hemolysin Expression
Journal of Microbiology and Biotechnology
2016 .01
-
Soluble Expression of Artificially Designed Recombinant Protein through Conjugating Fusion Protein in Escherichia coli
한국미생물학회 학술대회논문집
2017 .04
-
합성 유전자를 이용하여 Escherichia coli에서 백신 후보의 생산 혹은 진단용 항체의 개발을 위한 인간 rotavirus VP8* 부분 단백질의 발현
생명과학회지
2018 .04
-
Production of Recombinant Spider Silk Protein in Escherichia coli
한국미생물학회 학술대회논문집
2015 .04
-
Evolution of Escherichia coli for Optimal Growth in a Rich Medium
한국미생물학회 학술대회논문집
2019 .04
-
Effects of the Number of the Origin of Replication on the Cell Physiology of Escherichia coli
한국미생물학회 학술대회논문집
2016 .04
-
Redesign of an Intrinsically Disordered Peptide Tag for Enhanced Soluble Expression of Recombinant Proteins
한국미생물학회 학술대회논문집
2022 .10
이 논문의 저자 정보
최근 본 자료
댓글(0)
0