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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
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- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제20권 제2호
- 발행연도
- 2010.1
- 수록면
- 383 - 390 (8page)
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A newly isolated active producer of raw-starch-digesting amyloytic enzymes, Rhizopus microsporus var. chinensis CICIM-CU F0088, was screened and identified by morphological characteristics and molecular phylogenetic analyses. This fungus was isolated from the soil of Chinese glue pudding mill, and produced high levels of amylolytic activity under solid-state fermentation with supplementation of starch and wheat bran. Results of thin-layer chromatography showed there are two kinds of amyloytic enzymes formed by this strain, including one α-amylase and two glucoamylases.
It was found in the electron microscope experiments that the two glucoamylases can digest raw corn starch and have an optimal temperature of 70oC. These results signified that amyloytic enzymes secreted by strain Rhizopus microsporus var. chinensis CICIM-CU F0088 were types of thermostable amyloytic enzymes and able to digest raw corn starch.
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참고문헌 (22)
참고문헌 신청
Acevedo, E / 1997 / Regulation of α-amylase isoenzymes expression in Araucaria araucona by gibberellic and abscisic acids / Phytochemistry 44 : 1401 ~ 1405
Anderson, K / 2000 / Diphenylamineaniline- phosphoric acid reagent, a versatile spray reagent for revealing glycoconjugates on thin-layer chromatography plates / Anal. Bioche
Annunziato, M. E / 2006 / Production of α-galactosidase from Aspergilus oryzae grown in solid state culture / J. Food Sci 51 : 1370 ~ 1371
Chou, W. I / 2006 / The family 21 carbohydrate-binding module of glucoamylase from Rhizopus oryzae consists of two sites playing distinct roles in ligand binding / Biochem. J
Doyle, J. J / 1987 / A rapid DNA isolation procedure for small quantities of fresh leaf tissue / Phytochem. Bull 19 : 11 ~ 15
Anderson, K / 2000 / Diphenylamineaniline- phosphoric acid reagent, a versatile spray reagent for revealing glycoconjugates on thin-layer chromatography plates / Anal. Bioche
Annunziato, M. E / 2006 / Production of α-galactosidase from Aspergilus oryzae grown in solid state culture / J. Food Sci 51 : 1370 ~ 1371
Chou, W. I / 2006 / The family 21 carbohydrate-binding module of glucoamylase from Rhizopus oryzae consists of two sites playing distinct roles in ligand binding / Biochem. J
Doyle, J. J / 1987 / A rapid DNA isolation procedure for small quantities of fresh leaf tissue / Phytochem. Bull 19 : 11 ~ 15
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