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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제28권 제3호
- 발행연도
- 2018.1
- 수록면
- 409 - 417 (9page)
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초록· 키워드
오류제보하기
Biologically produced 2,3-butanediol (2,3-BDO) has diverse industrial applications. In this study, schematic isolation and screening procedures were designed to obtain generally regarded as safe (GRAS) and efficient 2,3-BDO producers. Over 4,000 candidate strains were isolated by pretreatment and enrichment, and the isolated Bacillus strains were further screened by morphological, biochemical, and genomic analyses. The screened strains were then used to test the utilization of the most common carbon (glucose, xylose, fructose, sucrose) and nitrogen (yeast extract, corn steep liquor) sources for the economical production of 2,3-BDO. Two-stage fed-batch fermentation was finally carried out to enhance 2,3-BDO production. In consequence, a newly isolated Bacillus licheniformis GSC3102 strain produced 92.0 g/l of total 2,3-BDO with an overall productivity and yield of 1.40 g/l/h and 0.423 g/g glucose, respectively, using a cheap and abundant nitrogen source. These results strongly suggest that B. licheniformis, which is found widely in nature, can be used as a host strain for the industrial fermentative production of 2,3-BDO.
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참고문헌 (38)
참고문헌 신청
Taylor MB / 1960 / Stereoisomeric specificities of 2,3-butanediol dehydrogenases / Biochim. Biophys. Acta 39:448~457
Ge L / 2011 / A new method for industrial production of 2,3-butanediol / J. Biomater. Nanobiotechnol. 2:335~336
Ji XJ / 2011 / Microbial 2,3-butanediol production: a state-of-the-art review / Biotechnol. Adv. 29:351~364
Ji XJ / 2010 / Engineering Klebsiella oxytoca for efficient 2,3-butanediol production through insertional inactivation of acetaldehyde dehydrogenase gene / Appl. Microbiol. Biotechnol. 85:1751~1758
Syu MJ / 2001 / Biological production of 2,3-butanediol / Appl. Microbiol. Biotechnol. 55:10~18
Ge L / 2011 / A new method for industrial production of 2,3-butanediol / J. Biomater. Nanobiotechnol. 2:335~336
Ji XJ / 2011 / Microbial 2,3-butanediol production: a state-of-the-art review / Biotechnol. Adv. 29:351~364
Ji XJ / 2010 / Engineering Klebsiella oxytoca for efficient 2,3-butanediol production through insertional inactivation of acetaldehyde dehydrogenase gene / Appl. Microbiol. Biotechnol. 85:1751~1758
Syu MJ / 2001 / Biological production of 2,3-butanediol / Appl. Microbiol. Biotechnol. 55:10~18
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