지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
Herbicide resistance is the most common trait being tested and thus herbicide‐resistant genetically modified plants are now the most widely cultivated worldwide. Here we developed herbicide‐resistant transgenic Agrostis mongolica Roshev. by employing an efficient Agrobacterium‐mediated transformation procedure with 25.2% of transformation efficiency. The identification and employment of regenerable and reproducible type of callus was one of the most critical factors to ensure success in this study. PCR analysis confirmed that the bar transgene was integrated into the genome of transgenic plants. The expression of 35S‐bar gene was confirmed by Northern blot analysis. The transgenic plants showed complete resistance to herbicide, indicating that the bar gene is functional in transgenic plants.
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참고문헌 (16)
참고문헌 신청
Asano Y / 1998 / Herbicide?resistant transgenic creeping bentgrass plants obtained by electroporation using an altered buffer / Plant Cell Rep 17 : 963 ~ 967
Belanger FC / 2003 / Interspecific hybridization between Agrostis stolonifera and related Agrostis species under field conditions.Crop Sci.43:240?246 / Crop Sci 43 : 240 ~ 24
Chai ML / 2003 / Agrobacterium?mediated transformation of herbicide resistance in creeping bentgrass and colonial bentgrass / J. Zhejiang Univ. Sci 4 : 346 ~ 351
Chai ML / 2004 / Transgenic plants of colonial bentgrass from embryogenic callus via Agrobacterium?mediated transformation / Plant Cell Tiss. Org. Cult 77 : 165 ~ 171
Enkhchimeg V / 2005 / Overexpression of Arabidopsis ABF3 gene enhances tolerance to drought and cold in transgenic lettuce (Lactuca sativa L.) / Plant Cell Tiss. Org. Cult 83
Belanger FC / 2003 / Interspecific hybridization between Agrostis stolonifera and related Agrostis species under field conditions.Crop Sci.43:240?246 / Crop Sci 43 : 240 ~ 24
Chai ML / 2003 / Agrobacterium?mediated transformation of herbicide resistance in creeping bentgrass and colonial bentgrass / J. Zhejiang Univ. Sci 4 : 346 ~ 351
Chai ML / 2004 / Transgenic plants of colonial bentgrass from embryogenic callus via Agrobacterium?mediated transformation / Plant Cell Tiss. Org. Cult 77 : 165 ~ 171
Enkhchimeg V / 2005 / Overexpression of Arabidopsis ABF3 gene enhances tolerance to drought and cold in transgenic lettuce (Lactuca sativa L.) / Plant Cell Tiss. Org. Cult 83
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