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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
Maize is the most important grain crop in the world. The genetic engineering technology has been used to enhance the various agronomical traits. The maize transformation is a crucial step for application of gene technologies to improve maize. The choice of genotype and explant material influences on the transformation efficiency and the production of stable transgenic plants. Immature embryos of Hi IIA were infected with Agrobacterium tumefaciens LBA4404 including superbinary vector (bar and GUS or GFP genes). The transformation efficiency was based on the transgenic calli induction from immature embryos on selection medium with 3 mg/L bialaphos. The transformation efficiency varied from 1.01 to 2.74%. The integration and expression of bar, GUS, and GFP genes were confirmed in T0 and T1 generations of transgenic plants using genomic PCR and bar strip test. Also herbicide resistance in T1 transgenic plants was shown when leaves and whole plants were treated with Basta. These results suggest that the successful Agrobacterium-mediated transformation of Hi IIA will improve further opportunities for maize functional genomic and genome editing studies.
목차
등록된 정보가 없습니다.
참고문헌 (30)
참고문헌 신청
Char SN / 2017 / An Agrobacterium-delivered CRISPR/Cas9system for high-frequency targeted mutagenesis in maize / Plant Biotech J 15:257~268
Coelho CM / 2005 / Cyclin-dependent kinase inhibitors in maize endosperm and their potential role in endoreduplication / Plant Physiol 138:2323~2336
조미애 / 2005 / Agrobacterium tumefaciens 공동배양법을 이용한 옥수수 형질전환체 생산 / Journal of Plant Biotechnology 32(2):91~95
Cho MJ / 2014 / Agrobacterium-mediated high-frequency transformation of an elite commercial maize(Zea mays L. )inbred line / Plant Cell Rep 33:1767~1777
Cho MJ / 2015 / Improvement of Agrobacterium-mediated transformation frequency in multiple modern elite commercial maize(Zea mays L. )inbreds by media modifications / Plant Cell Tiss Organ Cult 121:519~529
Coelho CM / 2005 / Cyclin-dependent kinase inhibitors in maize endosperm and their potential role in endoreduplication / Plant Physiol 138:2323~2336
조미애 / 2005 / Agrobacterium tumefaciens 공동배양법을 이용한 옥수수 형질전환체 생산 / Journal of Plant Biotechnology 32(2):91~95
Cho MJ / 2014 / Agrobacterium-mediated high-frequency transformation of an elite commercial maize(Zea mays L. )inbred line / Plant Cell Rep 33:1767~1777
Cho MJ / 2015 / Improvement of Agrobacterium-mediated transformation frequency in multiple modern elite commercial maize(Zea mays L. )inbreds by media modifications / Plant Cell Tiss Organ Cult 121:519~529
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