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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.12
- 수록면
- 397 - 405 (9page)
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초록· 키워드
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The Agrobacterium-mediated transient assay is a relatively rapid technique and a promising approach for assessing the expression of a gene of interest. Despite the successful application of this transient expression system in several plant species, it is not well understood in spinach.
In this study, we analyzed various factors, including infiltration method, Agrobacterium strain and density, and coinfiltration of an RNA silencing suppressor (p19), that affect transient expression following agroinfiltration in spinach. To evaluate the effects of these factors on the transient expression system, we used the b-glucuronidase (GUS) reporter gene construct pB7WG2D as a positive control. The vacuum-based infiltration method was much more effective at GUS gene expression than was the syringe- based infiltration method. Among the three Agrobacterium strains examined (EHA105, LBA4404, and GV2260), infiltration with the GV2260 strain suspension at a final optical cell density (OD600) of 1.0 resulted in the highest gene expression. Furthermore, co-expression of suppressor p19 also increased the efficiency and duration of gene expression and protein accumulation. The results indicate that the use of optimized conditions for transient gene expression could be a simple, rapid, and effective tool for functional genomics in spinach.
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등록된 정보가 없습니다.
참고문헌 (35)
참고문헌 신청
Anderson AR / 1979 / Host specificity in the genus Agrobacterium / Phytopathology 69(4):320~323
Avsar B / 2017 / In silico analysis of microRNAs in Spinacia oleracea genome and transcriptome / Int J Biosci Biochem Bioinform 7(2):84~92
Bakrim A / 2008 / Ecdysteroids in spinach (Spinacia oleracea L.): biosynthesis, transport and regulation of levels / Plant Physiol Biochem 46(10):844~854
Chin DP / 2009 / Transgenic spinach plants produced by Agrobacterium-mediated method based on the low temperature-dependent high plant regeneration ability of leaf explants / Plant Biotechnol 26(2):243~248
손성한 / 2008 / The Effect of Cucumber mosaic virus 2b Protein to Transient Expression and Transgene Silencing Mediated by Agro-infiltration / The Plant Pathology Journal 24(3):296~304
Avsar B / 2017 / In silico analysis of microRNAs in Spinacia oleracea genome and transcriptome / Int J Biosci Biochem Bioinform 7(2):84~92
Bakrim A / 2008 / Ecdysteroids in spinach (Spinacia oleracea L.): biosynthesis, transport and regulation of levels / Plant Physiol Biochem 46(10):844~854
Chin DP / 2009 / Transgenic spinach plants produced by Agrobacterium-mediated method based on the low temperature-dependent high plant regeneration ability of leaf explants / Plant Biotechnol 26(2):243~248
손성한 / 2008 / The Effect of Cucumber mosaic virus 2b Protein to Transient Expression and Transgene Silencing Mediated by Agro-infiltration / The Plant Pathology Journal 24(3):296~304
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