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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.1
- 수록면
- 83 - 87 (5page)
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Genetic transformation was affected by material of explant, age of callus, and medium of regeneration. Two rice seed cultivars (Ilpum and Baekjinju) and mediums were investigated in this study for enhancing regeneration of transgenic rice expressed AtBI-1 gene encoding the Arabidopsis thaliana Bax inhibitor. Regeneration rate of Ilpum rice transformant in gelrite of 5 and 8 g were 27.4% and 18.0%, respectively. In Baekjinju, regeneration rate of transformant was 5.4% and 4.3% in 5 and 8 g gelrite, respectively. The highest number of transformant plant in this study was regenerated from Ilpum cultivar on MS medium (30.4%) and was applied for the subsequent experiment. The callus regeneration rate of transformant were 40.7% in callus infection of up-side, it was higher regeneration then in the down-side (3.9%). The regeneration rate of callus of 25 days and 35 days were 14.7% and 38.6%, respectively. The most important application of this work is in genetic transformation of rice, particularly for improvement transgenic plant tissue culture protocol with high frequency of plant regeneration.
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참고문헌 (12)
참고문헌 신청
Cho MJ / 2003 / Stable transformation of rice (Oryza sativa L.) via microprojectile bombardment of highly regenerative, green tissues derived from mature seed / Plant Cell Re
Christou P / 1991 / Production of transgenic rice (Oryza sativa L.) plants from agronomically important Indica and Japonica varieties via electric discharge particle accelera
Datta SK / 1990 / Genetically engineered fertile Indica rice recovered from protoplasts / Nature Biotechnology 8 : 736 ~ 740
Dong J / 2001 / Characterization of rice transformed via an Agrobacterium-mediated inflorescence approach / Molecular Breeding 7 : 187 ~ 194
Heyser JW / 1983 / High frequency, long term regeneration of rice from callus culture / Plant Science Letters 29 : 175 ~ 182
Christou P / 1991 / Production of transgenic rice (Oryza sativa L.) plants from agronomically important Indica and Japonica varieties via electric discharge particle accelera
Datta SK / 1990 / Genetically engineered fertile Indica rice recovered from protoplasts / Nature Biotechnology 8 : 736 ~ 740
Dong J / 2001 / Characterization of rice transformed via an Agrobacterium-mediated inflorescence approach / Molecular Breeding 7 : 187 ~ 194
Heyser JW / 1983 / High frequency, long term regeneration of rice from callus culture / Plant Science Letters 29 : 175 ~ 182
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