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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.1
- 수록면
- 388 - 393 (6page)
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초록· 키워드
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We report an Agrobacterium-mediated transformation procedure optimized for ‘Kyoho’ that is a major table grapevine cultivar in Korea, and its transgenic plants with antifreeze protein gene of carrot (DcAFP). The full length of DcAFP coding region in accordance with the previous report was isolated from young leaves of carrot and recombined into a plant transformation vector. Ethylene inhibitors such as silver nitrate and aminoethoxyvinylglycin (AVG) supplemented in a co-cultivation medium distinctly increased frequency of shoot regeneration when explants were sub-cultured in a selection medium: particularly ten-fold higher in treatment with 0.1 mg/L AVG than one without ethylene inhibitor. Among various antibiotics and their concentrations, the combination of 150 mg/L cefotaxime plus 150 mg/L ClavamoxTM was selected for elimination of Agrobacterium cells in addition to minimization of adverse effect on shoot regeneration, while 50 mg/L kanamycin monosulfate effectively suppressed regeneration of nontransgenic shoots. Applying the elucidated culture condition, we finally obtained a total of 5 transgenic ‘Kyoho’ plantlets with DcAFP, of which integration with the grapevine genome and transcription was confirmed by nucleic acid analyses.
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참고문헌 (24)
참고문헌 신청
Alsheikh MK / 2002 / Appropriate choice of antibiotic and Agrobacterium strain improves transformation of antibiotic-sensitive Fragaria vesca and Fragaria vesca semperflorens
Beyer EM / 1979 / Effect of silver ion, carbon dioxide and oxygen on ethylene action and metabolism / Plant Physiol 63 : 169 ~ 173
Chang S / 1993 / A simple and efficient method for isolating RNA from pine trees / Plant Mol Biol Rep 11 : 113 ~ 116
Chi G-L / 1989 / Ethylene inhibitors enhanced de novo shoot regeneration from cotyledons of Brassica campestris ssp. chinensis (Chinese cabbage) in vitro / Plant Sci 64 : 243
DeVries AL / 1970 / Chemical and physical properties of freezing point-depressing glycoproteins from Antarctic fishes / J Biol Chem 245 : 2901 ~ 2908
Beyer EM / 1979 / Effect of silver ion, carbon dioxide and oxygen on ethylene action and metabolism / Plant Physiol 63 : 169 ~ 173
Chang S / 1993 / A simple and efficient method for isolating RNA from pine trees / Plant Mol Biol Rep 11 : 113 ~ 116
Chi G-L / 1989 / Ethylene inhibitors enhanced de novo shoot regeneration from cotyledons of Brassica campestris ssp. chinensis (Chinese cabbage) in vitro / Plant Sci 64 : 243
DeVries AL / 1970 / Chemical and physical properties of freezing point-depressing glycoproteins from Antarctic fishes / J Biol Chem 245 : 2901 ~ 2908
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