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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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Objectives: Glucagon-like peptide-1 (GLP-1) is an intestinally secreted hormone and it plays an important role in the regulation of glucose homeostasis. However, the possible role of GLP-1 in the differentiation of adipose-derived stem cells (ADSCs) remains unknown. Therefore this study investigated the effect of GLP-1 on the differentiation of ADSCs into osteoblasts and adipocytes. Methods: ADSCs were isolated from human adipose tissues of the abdomens, cultured and characterized by flow cytometry and multi-lineage potential assay. ADSCs were induced in osteogenic and adipogenic media treated with two different doses (10 and 100 nM) of GLP-1, and then the effect of GLP-1 on differentiation of ADSCs into osteoblast and adipocyte was examined. The signaling pathway involved in these processes was also examined. Results: Isolated human ADSCs expressed mesenchymal stem cell (MSC) specific markers as well as GLP-1 receptor (GLP-1R) proteins. They also showed multiple-lineage potential of MSC. GLP-1 was upregulated the activity and mRNA expression of osteoblast-specific marker, alkaline phosphatase and the mineralization of calcium. In contrast, GLP-1 significantly suppressed the expression of adipocyte-specific markers, peroxisome proliferator-activated receptor gamma (PPAR-γ), lipoprotein lipase (LPL) and adipocyte protein 2 (AP2). This decreased expression of adipocyte specific markers caused by GLP-1 was significantly reversed by the treatment of extracellular signal-regulated kinase (ERK) inhibitor, PD98059 (P < 0.05).Conclusion: This result demonstrates that GLP-1 stimulates osteoblast differentiation in ADSCs, whereas it inhibits adipocyte differentiation. The ERK signaling pathway seems to be involved in these differentiation processes mediated by GLP-1. (J Menopausal Med 2015;21:93-103)
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참고문헌 (25)
참고문헌 신청
Alvarez E. / 1996 / Expression of the glucagon-like peptide-1 receptor gene in rat brain / J Neurochem 66 : 920 ~ 927
Alvarez E. / 2005 / The expression of GLP-1 receptor mRNA and protein allows the effect of GLP-1 on glucose metabolism in the human hypothalamus and brainstem / J Neurochem 9
Barnett A. / 2006 / DPP-4 inhibitors and their potential role in the management of type 2 diabetes / Int J Clin Pract 60 : 1454 ~ 1470
Beresford JN. / 1992 / Evidence for an inverse relationship between the differentiation of adipocytic and osteogenic cells in rat marrow stromal cell cultures / J Cell Sci 1
Cowan CM. / 2004 / Adipose-derived adult stromal cells heal criticalsize mouse calvarial defects / Nat Biotechnol 22 : 560 ~ 567
Alvarez E. / 2005 / The expression of GLP-1 receptor mRNA and protein allows the effect of GLP-1 on glucose metabolism in the human hypothalamus and brainstem / J Neurochem 9
Barnett A. / 2006 / DPP-4 inhibitors and their potential role in the management of type 2 diabetes / Int J Clin Pract 60 : 1454 ~ 1470
Beresford JN. / 1992 / Evidence for an inverse relationship between the differentiation of adipocytic and osteogenic cells in rat marrow stromal cell cultures / J Cell Sci 1
Cowan CM. / 2004 / Adipose-derived adult stromal cells heal criticalsize mouse calvarial defects / Nat Biotechnol 22 : 560 ~ 567
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