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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.1
- 수록면
- 661 - 671 (11page)
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Adipose tissue-derived mesenchymal stem cells (ADSCs) are promising for regenerating degenerated intervertebral discs (IVDs), but the low efficiency of nucleus pulposus (NP)-specific differentiation limits their clinical applications. The Sonic hedgehog (Shh) signaling pathway is important in NP-specific differentiation of ADSCs, and Smoothened Agonist (SAG) is a highly specific and effective agonist of Shh signaling. In this study, we proposed a new differentiation strategy with the use of the small molecule SAG. The NP-specific differentiation and extracellular matrix (ECM) synthesis of ADSCs were measured in vitro, and the regenerative effects of SAG pretreated ADSCs in degenerated IVDs were verified in vivo. The results showed that the combination of SAG and transforming growth factor-β3 (TGF-β3) is able to increase the ECM synthesis of ADSCs. In addition, the gene and protein expression levels of NP-specific markers were increased by treatment with SAG and TGF-β3. Furthermore, SAG pretreated ADSCs can also improve the disc height, water content, ECM content, and structure of degenerated IVDs in vivo. Our new differentiation scheme has high efficiency in inducing NP-specific differentiation of ADSCs and is promising for stem cell-based treatment of degenerated IVDs.
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참고문헌 (55)
참고문헌 신청
Amaral, J. D. / 2019 / A novel small molecule p53 stabilizer for brain cell differentiation / Front. Chem 7:15
Amirpour, N. / 2019 / Differentiation of eye field neuroectoderm from human adipose-derived stem cells by using small-molecules and hADSCconditioned medium / Ann. Anat 221:17~26
Andersson, G. B. / 1999 / Epidemiological features of chronic low-back pain / Lancet 354:581~585
Briscoe, J. / 2015 / Morphogen rules : design principles of gradient-mediated embryo patterning / Development 142:3996~4009
Chen, J. / 2006 / Molecular phenotypes of notochordal cells purified from immature nucleus pulposus / Eur. Spine J 15(Suppl 3):S303~S311
Amirpour, N. / 2019 / Differentiation of eye field neuroectoderm from human adipose-derived stem cells by using small-molecules and hADSCconditioned medium / Ann. Anat 221:17~26
Andersson, G. B. / 1999 / Epidemiological features of chronic low-back pain / Lancet 354:581~585
Briscoe, J. / 2015 / Morphogen rules : design principles of gradient-mediated embryo patterning / Development 142:3996~4009
Chen, J. / 2006 / Molecular phenotypes of notochordal cells purified from immature nucleus pulposus / Eur. Spine J 15(Suppl 3):S303~S311
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