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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.1
- 수록면
- 282 - 290 (9page)
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초록· 키워드
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Cuphea viscosissima plants accumulate medium- chain fatty acids (MCFAs), i.e., those containing 8 ~ 14 carbons, in their seeds, in addition to the longer carbon chain fatty acids (≥16 carbons) found in a variety of plant species. Previous studies have reported the existence of three C. viscosissima MCFA-producing acyl-acyl carrier protein (ACP) thioesterases with different substrate specificities. In this study, CvFatB4, a novel cDNA clone encoding an acyl- ACP thioesterase (EC 3.1.2.14), was isolated from developing C. viscosissima seeds. Sequence alignment of the deduced amino acid sequence revealed that four catalytic residues for thioesterase activity are conserved and a putative N-terminal chloroplast transit peptide is present. Overexpression of CvFatB4 cDNA, which was under the control of the cauliflower mosaic virus 35S promoter, in Arabidopsis thaliana led to an increase in 16:0 fatty acid (palmitate) levels in the seed oil at the expense of 18:1 and other non-MCFAs.
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참고문헌 (23)
참고문헌 신청
Christopher Beermann / 2007 / Comparison of the Structures of Triacylglycerols from Native and Transgenic Medium-Chain Fatty Acid-Enriched Rape Seed Oil by Liquid Chromatography–Atmospheric Pressure Chemical Ionization Ion-Trap Mass Spectrometry (LC–APCI-ITMS) / Lipids 42(4):383~394
Steven J. Clough / 1998 / Floral dip: a simplified method forAgrobacterium-mediated transformation ofArabidopsis thaliana / The Plant Journal 16(6):735~743
K. G. Srikanta Dani / 2011 / Structural and functional analyses of a saturated acyl ACP thioesterase, type B from immature seed tissue of Jatropha curcas / Plant Biology 13(3):453~461
H.Maelor Davies / 1993 / Medium chain acyl-ACP hydrolysis activities of developing oilseeds / Phytochemistry 33(6):1353~1356
H.Maelor Davies / 1991 / Developmental induction, purification, and further characterization of 12:0-ACP thioesterase from immature cotyledons of Umbellularia californica / Archives of Biochemistry and Biophysics 290(1):37~45
Steven J. Clough / 1998 / Floral dip: a simplified method forAgrobacterium-mediated transformation ofArabidopsis thaliana / The Plant Journal 16(6):735~743
K. G. Srikanta Dani / 2011 / Structural and functional analyses of a saturated acyl ACP thioesterase, type B from immature seed tissue of Jatropha curcas / Plant Biology 13(3):453~461
H.Maelor Davies / 1993 / Medium chain acyl-ACP hydrolysis activities of developing oilseeds / Phytochemistry 33(6):1353~1356
H.Maelor Davies / 1991 / Developmental induction, purification, and further characterization of 12:0-ACP thioesterase from immature cotyledons of Umbellularia californica / Archives of Biochemistry and Biophysics 290(1):37~45
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