지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.1
- 수록면
- 572 - 576 (5page)
이용수
초록· 키워드
오류제보하기
The Luminex-based single antigen bead (SAB) assay is widely used to detect HLA antibody in transplant recipients. However, one limitation of the SAB assay is the prozone effect, which occurs mostly as a result of complement interference. We investigated the efficacy of EDTA treatment for overcoming the prozone effect and predicting C1q binding of HLA antibody. We subjected 27 non-treated (naïve) and EDTA-treated serum samples from highly sensitized patients to IgG-SAB assays, and we confirmed the prozone effect in 53% and 31% of class I and class II antibody tests, respectively, after EDTA treatment. When we conducted additional assays after dithiothreitol treatment and serum dilution, EDTA was the most efficacious in eliminating the prozone effect. Reducing the prozone effect by EDTA treatment strengthened the correlation between IgG mean fluorescence intensity (MFI) and C1q MFI values (ρ=0.825) as compared with the naïve sera (ρ=0.068). Although C1q positivity was dependent on the concentration of HLA antibody in EDTA-treated sera, the correlations varied individually. Overall, our results confirmed the efficacy of EDTA treatment for overcoming the prozone effect. EDTA treatment showed a positive effect on the correlation between IgG MFI and C1q MFI values.
목차
등록된 정보가 없습니다.
참고문헌 (20)
참고문헌 신청
Tambur AR / 2018 / Sensitization in transplantation : assessment of risk(STAR)2017 working group meeting report / Am J Transplant 18:1604~1614
오은지 / 2015 / Interlaboratory Comparison of the Results of Lifecodes LSA Class I and Class II Single Antigen Kits for Human Leukocyte Antigen Antibody Detection / Annals of Laboratory Medicine 35(3):321~328
Gebel HM / 2010 / The evolution and clinical impact of human leukocyte antigen technology / Curr Opin Nephrol Hypertens 19:598~602
장지영 / 2012 / Application of Calculated Panel Reactive Antibody Using HLA Frequencies in Koreans / Annals of Laboratory Medicine 32(1):66~72
민지원 / 2018 / Clinical Impact of Pre-transplant Antibodies Against Angiotensin II Type I Receptor and Major Histocompatibility Complex Class I-Related Chain A in Kidney Transplant Patients / Annals of Laboratory Medicine 38(5):450~457
오은지 / 2015 / Interlaboratory Comparison of the Results of Lifecodes LSA Class I and Class II Single Antigen Kits for Human Leukocyte Antigen Antibody Detection / Annals of Laboratory Medicine 35(3):321~328
Gebel HM / 2010 / The evolution and clinical impact of human leukocyte antigen technology / Curr Opin Nephrol Hypertens 19:598~602
장지영 / 2012 / Application of Calculated Panel Reactive Antibody Using HLA Frequencies in Koreans / Annals of Laboratory Medicine 32(1):66~72
민지원 / 2018 / Clinical Impact of Pre-transplant Antibodies Against Angiotensin II Type I Receptor and Major Histocompatibility Complex Class I-Related Chain A in Kidney Transplant Patients / Annals of Laboratory Medicine 38(5):450~457
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
HLA Class II 항체 검출 시 C3d 검사법과 SAB 검사법의 상관관계 평가
Laboratory Medicine Online
2020 .01
-
Interlaboratory Comparison of the Results of Lifecodes LSA Class I and Class II Single Antigen Kits for Human Leukocyte Antigen Antibody Detection
Annals of Laboratory Medicine
2015 .01
-
Study on comparison of metal toxicity on green algae in the absence and presence of EDTA
환경독성보건학회 심포지엄 및 학술대회
2020 .09
-
Enhancing the Antibacterial Effect of Erythrosine-Mediated Photodynamic Therapy with Ethylenediamine Tetraacetic Acid
대한소아치과학회지
2024 .02
-
Causes of Positive Pretransplant Crossmatches in the Absence of Donor-Specific Anti-Human Leukocyte Antigen Antibodies: A Single-Center Experience
Annals of Laboratory Medicine
2021 .01
-
Two Cases of Antibody-Mediated Rejection Following Kidney Transplantation due to HLA-DQB1 Allele-Specific and DQ Alpha Protein-Specific HLA Antibodies
Annals of Laboratory Medicine
2017 .01
-
False Positive Class II HLA Antibody Reaction Due to Antibodies Against Denatured HLA Might Differ Between Assays: One Lambda vs. Immucor
Annals of Laboratory Medicine
2020 .01
-
심장이식 후 동종이식 거부반응을 보인 소아 환자의 non-HLA 항체와 epitope 분석
Laboratory Medicine Online
2024 .04
-
Luminex를 이용한 이식면역검사
Clinical Transplantation and Research
2015 .01
-
Establishment of Reference Values for Non-HLA Antibodies in Patients With End-stage Renal Disease
Annals of Laboratory Medicine
2023 .03
-
Suitability of EDTA-anticoagulated Blood for Natural Killer Cell Activity Testing Using Flow Cytometry
Annals of Laboratory Medicine
2023 .05
-
Metastasis-Free Interval Is Closely Related to Tumor Characteristics and Has Prognostic Value in Breast Cancer Patients with Distant Relapse
Journal of Breast Cancer
2015 .01
-
Smear layer removal by passive ultrasonic irrigation and 2 new mechanical methods for activation of the chelating solution
Restorative Dentistry and Endodontics
2021 .01
-
Mineral content analysis of root canal dentin using laser-induced breakdown spectroscopy
Restorative Dentistry and Endodontics
2018 .01
-
Natural soluble human leukocyte antigen class I in donor serum neutralizes donor-specific HLA alloantibodies in recipient serum
Blood Research
2020 .01
-
Bortezomib-Containing Multimodality Treatment for Antibody-Mediated Rejection with Anti-HLA and Anti-AT1R Antibodies after Kidney Transplantation
Yonsei Medical Journal
2017 .01
-
Clinical significance of the pre-transplant HLA donor-specific antibody in liver transplantation
대한외과학회 학술대회 초록집
2019 .10
-
Anti-Alpha-Toxin Antibody Responses and Clinical Outcomes of Staphylococcus aureus Bacteremia
Journal of Korean Medical Science
2023 .04
-
Effect of phytic acid as an endodontic chelator on resin adhesion to sodium hypochlorite-treated dentin
Restorative Dentistry and Endodontics
2020 .01
-
Proposal of an Appropriate Decalcification Method of Bone Marrow Biopsy Specimens in the Era of Expanding Genetic Molecular Study
Journal of Pathology and Translational Medicine
2015 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0