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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2020.1
- 수록면
- 27 - 32 (6page)
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Background: Mutations in the quinolone resistance-determining regions (QRDRs) of Acinetobacter baumannii DNA gyrase (gyrA) and topoisomerase IV (parC) are linked to fluoroquinolone (FQ) resistance. We developed a mismatched PCR-restriction fragment length polymorphism (RFLP) assay to detect mutations in the gyrA and parC QRDRs associated with FQ resistance in A. baumannii. Methods: Based on the conserved sequences of A. baumannii gyrA and parC, two primer sets were designed for mismatched PCR-RFLP to detect mutations in gyrA (codons 83 and 87) and parC (codons 80 and 84) by introducing an artificial restriction enzyme cleavage site into the PCR products. This assay was evaluated using 58 A. baumannii strains and 37 other Acinetobacter strains that have been identified by RNA polymerase β-subunit gene sequence analysis. Results: PCR amplification of gyrA and parC was successful for all A. baumannii strains. In 11 FQ -susceptible strains, the gyrA and parC PCR products were digested by the selected restriction enzymes at the site containing gyrA (codons 83 and 87) and parC (codons 80 and 84). PCR products from 47 FQ-resistant strains containing mutations in gyrA and parC were not digested by the restriction enzymes at the site containing the mutation. As for the non-baumannii Acinetobacter strains, although amplification products for gyrA were obtained for 28 strains, no parC amplification product was obtained for any strain. Conclusions: This assay specifically amplified gyrA and parC from A. baumannii and detected A. baumannii gyrA and parC mutations with FQ resistance.
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참고문헌 (23)
참고문헌 신청
Hooper DC / 1998 / Clinical applications of quinolones / Biochim Biophys Acta 1400:45~61
김의석 / 2014 / Clinical Importance and Epidemiology of Quinolone Resistance / Infection and Chemotherapy 46(4):226~238
Dalhoff A / 2012 / Resistance surveillance studies : a multifaceted problem--the fluoroquinolone example / Infection 40:239~262
Gaynes R / 2005 / National Nosocomial Infections Surveillance System. Overview of nosocomial infections caused by gram-negative bacilli / Clin Infect Dis 41:848~854
Wisplinghoff H / 2004 / Nosocomial bloodstream infections in US hospitals: analysis of 24,179 cases from a prospective nationwide surveillance study / Clin Infect Dis 39:309~317
김의석 / 2014 / Clinical Importance and Epidemiology of Quinolone Resistance / Infection and Chemotherapy 46(4):226~238
Dalhoff A / 2012 / Resistance surveillance studies : a multifaceted problem--the fluoroquinolone example / Infection 40:239~262
Gaynes R / 2005 / National Nosocomial Infections Surveillance System. Overview of nosocomial infections caused by gram-negative bacilli / Clin Infect Dis 41:848~854
Wisplinghoff H / 2004 / Nosocomial bloodstream infections in US hospitals: analysis of 24,179 cases from a prospective nationwide surveillance study / Clin Infect Dis 39:309~317
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