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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.1
- 수록면
- 611 - 620 (10page)
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초록· 키워드
오류제보하기
Background: Vernix caseosa (VC), which is known as a unique human substance, is a biofilm that covers the skin of most human newborns. VC has many biological functions including anti-infective, skin cleansing and skin barrier repair. Objective: In the study, we purpose to investigate the novel effect of lipids extracted from VC on the regulation of filaggrin (FLG) expression and anti-inflammation in normal human epidermal keratinocyte (NHEK) cells. Methods: The lipids were extracted by chloroform/methanol (Folch method) and the major properties of fatty acid methyl esters were determined with gas chromatography-mass spectrometer. The relative viability of NHEK cells was evaluated by Cell Counting Kit 8 assay. The related expression of skin barrier protein was accessed with real-time quantitative polymerase chain reaction, Western blot and Immunofluorescence in NHEK cells with or without poly (I:C). Meanwhile, the changes of thymic stromal lymphopoietin (TSLP) and tumor necrosis factor alpha (TNF-α) are analyzed by enzyme- linked immunosorbent assay. Results: VC lipids mostly contained saturated and branched chains fatty acids. The expression of mRNA and protein of FLG were significantly increased after the supplement with lipid in NHEK cells. Meanwhile, lipids reversed the inhibition of poly (I:C) on FLG. Moreover, lipids suppressed the over secretion of TSLP and TNF-α induced by poly (I:C). Conclusion: These results indicate that lipids extracted from VC has positive effects on the expression of FLG and anti-inflammation, suggesting that lipids of VC may be used for a reference for novel therapeutic method in reducing and remedying skin disease like atopic disease.
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참고문헌 (37)
참고문헌 신청
Yamazaki Y / 2017 / Role of the microbiota in skin immunity and atopic dermatitis / Allergol Int 66:539~544
Palmer CN / 2006 / Common loss-of-function variants of the epidermal barrier protein filaggrin are a major predisposing factor for atopic dermatitis / Nat Genet 38:441~446
Brown SJ / 2012 / One remarkable molecule: filaggrin / J Invest Dermatol 132(3 Pt 2):751~762
Irvine AD / 2011 / Filaggrin mutations associated with skin and allergic diseases / N Engl J Med 365:1315~1327
Brandt EB / 2011 / Th2 cytokines and atopic dermatitis / J Clin Cell Immunol 2:110
Palmer CN / 2006 / Common loss-of-function variants of the epidermal barrier protein filaggrin are a major predisposing factor for atopic dermatitis / Nat Genet 38:441~446
Brown SJ / 2012 / One remarkable molecule: filaggrin / J Invest Dermatol 132(3 Pt 2):751~762
Irvine AD / 2011 / Filaggrin mutations associated with skin and allergic diseases / N Engl J Med 365:1315~1327
Brandt EB / 2011 / Th2 cytokines and atopic dermatitis / J Clin Cell Immunol 2:110
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