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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
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A eukaryotic microalga was isolated from seawater in Janghang Harbor, Korea and its morphological, molecular, and physiological characteristics were investigated. Due to its simple morphology, no distinctive characters were found by morphological observation, such as light microscope or scanning/transmission electron microscopy (S/TEM). However, molecular phylogenetic evidence inferred from the concatenated small subunit (SSU) 18S rRNA and internal transcribed spacer (ITS) sequence data indicated that the isolate belonged to the newly described Micractinium singularis. Furthermore, it was clustered with Antarctic Micractinium strains and it also showed a psychrotolerant property, surviving at temperatures as low as 5°C. However, its optimal growth temperatures range from 15°C to 25°C, indicating that this microalga is a mesophile. Additionally, gas chromatography-mass spectrometry (GC/MS) analysis showed that the isolate was rich in nutritionally important omega-3 polyunsaturated fatty acid, and high-performance liquid chromatography analysis (HPLC) revealed that the high-value antioxidant lutein was biosynthesized as an accessory pigment by this microalga, with glucose as the major monosaccharide. Therefore, in this study, a Korean marine M. singularis species was discovered, characterized, and described. It was subsequently added to the national culture collections.
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참고문헌 (33)
참고문헌 신청
Ausich RL / 1997 / Commercial opportunities for carotenoid production by biotechnology / Pure Appl. Chem 69:2169~2173
Boelen P / 2013 / On the potential application of polar and temperate marine microalgae for EPA and DHA production / AMB Express 3:1~9
Breuer G / 2013 / Analysis of fatty acid content and composition in microalgae / J. Vis. Exp 80:e50628
Buscemi S / 2018 / The effect of lutein on eye and extra-eye health / Nutrients 10:1321
Hyunsik Chae / 2019 / Morphology and phylogenetic relationships of Micractinium (Chlorellaceae, Trebouxiophyceae) taxa, including three new species from Antarctica / ALGAE 34(4):267~275
Boelen P / 2013 / On the potential application of polar and temperate marine microalgae for EPA and DHA production / AMB Express 3:1~9
Breuer G / 2013 / Analysis of fatty acid content and composition in microalgae / J. Vis. Exp 80:e50628
Buscemi S / 2018 / The effect of lutein on eye and extra-eye health / Nutrients 10:1321
Hyunsik Chae / 2019 / Morphology and phylogenetic relationships of Micractinium (Chlorellaceae, Trebouxiophyceae) taxa, including three new species from Antarctica / ALGAE 34(4):267~275
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