Ginsenoside $R_e$ Increases Fertile and Asthenozoospermic Infertile Human Sperm Motility by Induction of Nitric Oxide Synthase

Zhang Hong; Zhou Qing-Ming; Li Xiao-Da; Xie Yi; Duan Xin; Min Feng-Ling; Liu Bing; Yuan Zhi-Gang

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자료유형: 학술저널

저자정보: Zhang Hong (Department of Medical Physics, Institute of Modern Physics, The Chinese Academy of Sciences) Zhou Qing-Ming (Department of Medical Physics, Institute of Modern Physics, The Chinese Academy of Sciences) Li Xiao-Da (Department of Medical Physics, Institute of Modern Physics, The Chinese Academy of Sciences) Xie Yi (Department of Medical Physics, Institute of Modern Physics, The Chinese Academy of Sciences) Duan Xin (Department of Medical Physics, Institute of Modern Physics, The Chinese Academy of Sciences) Min Feng-Ling (Department of Medical Physics, Institute of Modern Physics, The Chinese Academy of Sciences) Liu Bing (Department of Medical Physics, Institute of Modern Physics, The Chinese Academy of Sciences) Yuan Zhi-Gang (Department of Medical Physics, Institute of Modern Physics, The Chinese Academy of Sciences)

저널정보: 대한약학회 Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea 제29권 제2호

발행연도: 2006.1

수록면: 145 - 151 (7page)

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We investigated the effects of Ginsenoside $R_e$ on human sperm motility in fertile and asthenozoospermic infertile individuals in vitro and the mechanism by which the Ginsenosides play their roles. The semen samples were obtained from 10 fertile volunteers and 10 asthenozoospermic infertile patients. Spermatozoa were separated by Percoll and incubated with 0, 1, 10 or $100\;{\mu}M$ of Ginsenoside $R_e$. Total sperm motility and progressive motility were measured by computer-aided sperm analyzer (CASA). Nitric oxide synthase (NOS) activity was determined by the $^{3}H$-arginine to $^{3}H$-citrulline conversion assay, and the NOS protein was examined by the Western blot analysis. The production of sperm nitric oxide (NO) was detected using the Griess reaction. The results showed that Ginsenoside $R_e$ significantly enhanced both fertile and infertile sperm motility, NOS activity and NO production in a concentration-dependent manner. Sodium nitroprusside (SNP, 100 nM), a NO donor, mimicked the effects of Ginsenoside $R_e$. And pretreatment with a NOS inhibitor $N^{w}$-Nitro-L-arginine methyl ester (L-NAME, $100\;{\mu}M$) or a NO scavenger N-Acetyl-L-cysteine (LNAC, 1 mM) completely blocked the effects of Ginsenoside $R_e$. Data suggested that Ginsenoside $R_e$ is beneficial to sperm motility, and that induction of NOS to increase NO production may be involved in this benefit.

#Sperm motility #Nitric oxide synthase #Nitric oxide #Reactive oxygen species

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