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논문 기본 정보

자료유형
학술저널
저자정보
Kim, Joon-Chul (Division of Biological Science, College of Natural Sciences, Kangwon National University) Chang, Mi-Young (Division of Biological Science, College of Natural Sciences, Kangwon National University) Son, Su-In (Division of Biological Science, College of Natural Sciences, Kangwon National University) Heo, Su-Jeong (Regional Crop Experiment Station, Kangwon Agricultural Research and Extension Services)
저널정보
한국식물학회 식물학회지 식물학회지 제44권 제4호
발행연도
2001.1
수록면
224 - 230 (7page)

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To maintain embryogenic cell lines of Pimpinella brachycarpa, we suspension-cultured friable and vapidly growing yellowish calli in an MS liquid medium containing 0.2 $\mu$M 2,4-D and 0.5 $\mu$M BAP. Efficient somatic embryogenesis was achieved when selected tells were then transferred to an MS medium (0.2% gelrite) that contained 0.2 $\mu$M 2,4-D, 0.5 $\mu$M BAP and 10.0 $\mu$M TDZ (thidiazuron). These cells were cultured at 27$^{\circ}C$ under continuous illumination (21.5 $\mu$E m$^{-2}$ /s$^{-1}$ ). Embryogenic calli expanded about four-fold, and developed into pale yellow calli. Somatic embryogenesis was initiated only from glossy and nodular-type calli. After two more weeks of culture, globular embryos appeared on the surface of calli grown in the MS medium that contained 10.0 $\mu$M TDZ only or in combination with 0.5 $\mu$M NAA. Experimenting with 2,4-D, an auxin, to promote embryogenic calli resulted in excessive browning and death. We overcame this problem by growing glossy embryogenic and nodular calli on media that contained 10.0 $\mu$M TDZ. Calli that were not treated with TDI turned dark brown and were not viable. Up to 14% of th2 calli showed somatic embryos when the medium was supplemented with 10.0 $\mu$M TDZ and 0.5 $\mu$M NAA. Embryos from these TDZ-induced, somatic embryogenic calli grew efficiently forming multiple shoots and developing into normal plants. Therefore, efficient differentiation of suspension-cultured tell clusters into embryogenic calli, along with treatment of subsequent somatic embryos by TDZ, suggests that TDZ probably helps in establishing the optimum cytokinin-auxin ratio required for induction and expression of somatic embryogenesis.

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