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자료유형
학술저널
저자정보
Trabelsi, El Bahri (Laboratory of Plant Biology and Biotechnology, Faculty of Sciences of Tunis Campus Universitaire, Institut de lolivier de Tunisie, Rue Hedi Karray) Bouzid, Sadok (Laboratory of Plant Biology and Biotechnology, Faculty of Sciences of Tunis Campus Universitaire) Bouzid, Monji (Institut de lolivier de Tunisie, Rue Hedi Karray) Elloumi, Nedra (National Institute of Agronomic Research of Tunisia, Rue Hedi Karray) Belfeleh, Zina (National Institute of Agronomic Research of Tunisia, Rue Hedi Karray) Benabdallah, Abdallah (National Institute of Agronomic Research of Tunisia, Rue Hedi Karray) Ghezel, Rachida (National Institute of Agronomic Research of Tunisia, Rue Hedi Karray)
저널정보
한국식물학회 식물학회지 식물학회지 제46권 제3호
발행연도
2003.1
수록면
173 - 180 (8page)

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We induced somatic embryogenesis from the cotyledon segments of Olea europaea (L.) cvs. 'Chetoui', 'Chemleli', and 'Arbequina'. Calli were established from all three cultivars on OMc media supplemented with IBA and 2i-P. The greatest success was obtained with media that contained zero or low concentrations of growth regulators. High levels of hormones (i.e.,>0.5 mgL$^{-1}$ IRA and 2i-P) inhibited embryogenesis. Embryos at different maturation stages were observed with continuously proliferating secondary embryogenesis. Abnormally shaped embryos and teratoma were also noted. Four weeks was the optimal incubation period for inducing embryogenesis on the auxin-containing medium. In addition, 30 to 40 gL$^{-1}$ sucrose was more effective than glucose in stimulating the growth and maturation of somatic embryos. Embryogenic efficiency was also higher when multivariate combinations of nitrogen sources (inorganic and organic nitrogen forms) were used. The plantlets that were derived from our germinating somatic embryos were similar to those obtained from axillary buds.

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